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Microarray Gene Expression Analysis #1

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Luming-L opened this issue Jan 31, 2020 · 0 comments

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Luming-L commented Jan 31, 2020 •

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Intensity: relative expression level of the gene

Experiment

isolate RNA
label
hybridized to the arrays
washed
scan the intensity of the fluorescent dyes (hybridized target-probes)
analyze the grayscale image to identify the array spots and to measure their relative fluorescence intensities

Microarray Gene Expression Analysis Pipeline

Microarray Quantitation

Quality Control

Normalization

Scaling by Grid Position
Intensity Dependent Bias
Log Transformation
Per-Chip Scaling
Quantile Normalization

Exploratory Analysis (Gene Annotation, Sample Annotation)

Statistical Analysis

Functional Analysis

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