RELEASE_NOTES.txt

RELEASE NOTES FOR HICUP v0.9.2 (16 February 2023)
-------------------------------------------------

HiCUP v0.9.2 is a minor update incorporating the change described below:

- Updated documentation



RELEASE NOTES FOR HICUP v0.9.1 (16 February 2023)
-------------------------------------------------

HiCUP v0.9.1 is a minor update incorporating the change described below:

- hicup2homer conversion script is now executable.

- fixed bug in scribl_capture_ditag_sorter.pl caused by non-Linux line 
  endings.  The script now runs.



RELEASE NOTES FOR HICUP v0.9.0 (14 February 2023)
-------------------------------------------------

HiCUP v0.9.0 is a major update incorporating the change described below:

- hicup2juicer script may now be passed the parameter --digest which 
  enables users to retrieve the fragment ID instead of the dummy values 
  0 and 1 (contributed by https://github.com/lldelisle)

- hicup2juicer script may be passed the parameter --usemid which results 
  in the position in the middle of the restriction fragment being 
  reported instead of the 5' end 
  (contributed by https://github.com/lldelisle).

- Added scripts for processing SCRiBL datasets.

- Updated documentation.



RELEASE NOTES FOR HICUP v0.8.3 (11 January 2022)
-------------------------------------------------

HiCUP v0.8.3 is a minor update incorporating the change described below:

- Updated contact details



RELEASE NOTES FOR HICUP v0.8.2 (13 JULY 2021)
-------------------------------------------------

HiCUP v0.8.2 is a minor update incorporating the change described below:

- The get_captured_reads script has been renamed to hicup_capture. The
script is found in the Misc folder and is used to identify "captured"
di-tags in a Capture Hi-C experiment.  The renamed script now works in
both the Misc directory or in the same directory as hicup_module (which 
is useful for Conda installs or when creating symbolic links to the 
hicup_capture script).



RELEASE NOTES FOR HICUP v0.8.1 (16 FEBRUARY 2021)
-------------------------------------------------

HiCUP v0.8.1 is a minor update incorporating the change described below:

- HiCUP previously could not process Gzipped files when run on a Mac 
computer.  While HiCUP is designed for Linux systems, a simple fix was 
made on how Gzipped files are uncompressed and consequently HiCUP should 
now work on Mac systems.



RELEASE NOTES FOR HICUP v0.8.0 (08 OCTOBER 2020)
------------------------------------------------

HiCUP v0.8.0 is a major update incorporating the changes described below:

- HiCUP has a new script named hicup_reporter that collates the summary
reports from each step of the pipeline into a new report.

Note: This hicup_reporter script can be run on its own to collate summary
results files generated previously by HiCUP.

- The pipeline generates a new HTML summary report using the script
r_scripts/hicup_reporter.rmd (this script is executed by hicup_reporter).

Note: this requires the R packages Tidyverse and Plotly to be installed.



RELEASE NOTES FOR HICUP v0.7.4 (18 June 2020)
---------------------------------------------

HiCUP v0.7.4 is a minor update incorporating the changes described below:

-Fixed bug when specifying a cut-site containing an N (any nucleotide).  
The script hicup_truncater used to incorporate such Ns into the FASTQ 
truncated read.  This is now fixed and no longer occurs.

-HiCUP now uses CIGAR string information when positioning reads to 
restriction fragments and during the de-duplication process.

-Added scripts for GitHub Actions unit testing.

-Added a HiCUP Singularity recipe to the Misc folder.



RELEASE NOTES FOR HICUP v0.7.3 (13 May 2020)
--------------------------------------------

HiCUP v0.7.3 is a minor update incorporating the changes described below:

-Added hicup2juicer to make HiCUP output compatible with Juicer.

-Modification to hicup_deduplicator output so files strictly adhere to 
SAM/BAM format.

-Fixed bug preventing --nofill option from working.

-No longer reports Bowtie2 message concerning gzbuffer changes.



RELEASE NOTES FOR HICUP v0.7.2 (15 January 2019)
------------------------------------------------

HiCUP v0.7.2 is a minor update incorporating the changes described below:

-Fixed bug causing output files to not strictly adhere to SAM/BAM format. 



RELEASE NOTES FOR HICUP v0.7.1 (12 November 2018)
-------------------------------------------------

HiCUP v0.7.1 is a minor update incorporating the changes described below:

-Added the option --arima to HiCUP Digester, to generate digest files 
compatible with the Arima protocol.



RELEASE NOTES FOR HICUP v0.7.0 (26 October 2018)
------------------------------------------------

HiCUP v0.7.0 is a major update incorporating the changes described below:

HiCUP can now process restriction enzymes that cut at different sites.
Such sequences contain an "N" within the restriction enzyme recognition
site to denote the four bases (AGCT).

Thanks to Rola Dali, Edouard Henrion and Mathieu Bourgey (McGill University) 
for adding this feature. 



RELEASE NOTES FOR HICUP v0.6.1 (10 May 2018)
----------------------------------------------

HiCUP v0.6.1 is a minor update incorporating the changes described below:

-Bowtie2 may now align reads using more than one thread
(while preserving the input/output read order), thereby reducing 
processing times.  The number of threads allocated to Bowtie2 will be 
the number of threads specified by the user divided by the number of 
files processed. 

-Fixed bug causing HiCUP Mapper to try to read beyond the end of a mapped 
reads SAM file, consequently causing the script to crash.


RELEASE NOTES FOR HICUP v0.6.0 (23 April 2018)
----------------------------------------------

HiCUP v0.6.0 is a major update incorporating the changes described below:

-Parameters adjusted for HiCUP mapper in determining what constitutes a
multi-mapping read, when using Bowtie2 as the aligner. 


RELEASE NOTES FOR HICUP v0.5.10 (26 October 2017)
-------------------------------------------------

HiCUP v0.5.10 is a minor update incorporating the changes described below:

-Fixed bug causing hicup_mapper to not pair a small proportion of valid 
reads.


RELEASE NOTES FOR HICUP v0.5.9 (15 September 2016)
--------------------------------------------------

HiCUP v0.5.9 is a minor update incorporating the changes described below:

-Modified HiCUP Deduplicator so it may process genomes comprising
hundreds of chromosomes (e.g. genome assemblies)

-Added script Misc/get_captured_reads for identifying on/off target
di-tags in capture Hi-C datasets

-Fixed bug when running hicup_truncater independently, causing the script 
to interpret NoFill:0 in the config file as effectively NoFill:1


RELEASE NOTES FOR HICUP v0.5.8 (19 October 2015)
------------------------------------------------

HiCUP v0.5.8 is a minor update incorporating the changes described below:

- Fixed bug preventing an odd number of files being specified when using
a configuration file with hicup_digester, hicup_filter or 
hicup_deduplicator.


RELEASE NOTES FOR HICUP v0.5.7 (18 June 2015)
---------------------------------------------

HiCUP v0.5.7 is a minor update incorporating the changes described below:

- Fixed bug preventing whole pipeline running to completion if --outdir
is set to a folder other than the current working directory.


RELEASE NOTES FOR HICUP v0.5.6 (09 June 2015)
---------------------------------------------

HiCUP v0.5.6 is a minor update incorporating the changes described below:

- Fixed bug preventing, when multiple samples are processed, the 
generation of HTML summary reports and the text file auditing the 
progress of read pairs through the entire pipeline.


RELEASE NOTES FOR HICUP v0.5.5 (20 May 2015)
--------------------------------------------

HiCUP v0.5.5 is a minor update incorporating the changes described below:

- HiCUP produces a text file auditing the progress of read pairs through 
the entire pipeline for all samples processed.

- Added the 'Conversion' folder, containing scripts for converting HiCUP 
BAM/SAM output into formats compatible with GOTHiC, Homer, Hicpipe and 
Fit-Hi-C.

Fixed bug preventing users specifying two --re1 restriction enzymes 
(i.e. the enzymes that create the Hi-C ligation junctions).


RELEASE NOTES FOR HICUP v0.5.4 (01 April 2015)
----------------------------------------------
HiCUP v0.5.4 is a minor update incorporating the changes described below:

- HiCUP Digester checks the first line of each input file begins with a 
valid FASTA header.

- Fixed bug causing HiCUP to ignore choice of aligner.

- HiCUP output compatible with SamTools release (v1.2), which is more 
strict with regard to the PG headers in a BAM/SAM file.

- Fixed bug causing files to be compressed if zip:0 selected in a 
configuration file.

- Fixed bug causing --re2 option sequence (i.e. not --re1 sequence) to 
be used to truncate reads prior to mapping.

- Should one or more sample contain no valid di-tags the pipeline would 
produce no HTML summary files, even for samples containing valid di-tags. 
This no longer happens, instead the script displays a warning message 
reporting samples not progressing through the pipeline and produces 
HTML for samples containing valid di-tags.

- Improved documentation by creating Markdown files.  These were added to 
the 'Documentation' folder.  The HiCUP Manual and QuickStart 
PDF files were deleted.


RELEASE NOTES FOR HICUP v0.5.3 (31 December 2014)
-------------------------------------------------
HiCUP v0.5.3 is a minor update incorporating the changes described below:

- HiCUP attempts to intelligently name files as the pipeline proceeds.
Please Note: To avoid passing HiCUP input files with identical names 
prior to the filename extension.  For example, the files 'sample.fa' and 
'sample.fastq' would produce files with identical names as the pipeline 
progresses.  This problem could be overcome by renaming one the files to 
'sample2.fa'.   To minimise inconvenience, HiCUP will immediately 
produce a warning message and not run if the input filenames are too 
similar.


RELEASE NOTES FOR HICUP v0.5.2 (22 November 2014)
-------------------------------------------------
HiCUP v0.5.2 is a minor update incorporating the changes described below:

- When running the whole pipeline, a folder may be specified to which all 
intermediate files are written. These files are deleted after the 
hicup_deduplicator script has terminated.

- FASTQ format now specified differently, valid options are:
Sanger, Solexa_Illumina_1.0, Illumina_1.3 or Illumina_1.5

- All configuration files kept in the 'config_files' folder.

- hicup_digester may be passed lowercase characters denoting the 
restriction enzyme recognition site.

- Pipeline no longer terminates if SAM Tools in not installed.


RELEASE NOTES FOR HICUP v0.5.1 (02 November 2014)
-------------------------------------------------
HiCUP v0.5.1 is a minor update incorporating the changes described below:

- hicup_deduplicator now accepts --threads as an argument.


RELEASE NOTES FOR HICUP v0.5.0 (01 October 2014)
-------------------------------------------------
HiCUP v0.5.0 is a major update incorporating the changes described below:

- HiCUP compatible with Bowtie2.

- To reduce mis-mapping, HiCUP no longer uses --strata as a parameter 
when aligning with Bowtie.

- Perl module GD::Graph is no longer a dependency since the pipeline now 
uses R to generate figures.

- The scripts ‘hicup’, ‘hicup_trunacter’ and ‘hicup_mapper’ all require 
file pairs as input. Such pairs should now be placed on adjacent lines 
in their respective configuration files. (Previous versions of HiCUP 
required paired files to be placed on the same line, separated using 
the pipe (‘|’) character. HiCUP still accepts filename pairs in this 
deprecated format.)

- Command line arguments can be used in conjunction with a configuration 
file. Parameters set using the command line will override those set 
using a configuration file.

- Fixed bug causing HiCUP only being able to process files in the 
current working directory.


RELEASE NOTES FOR HICUP v0.4.2 (27 November 2013)
-------------------------------------------------
HiCUP v0.4.2 is a minor update incorporating the changes described below:

- Pipeline produces di-tag length (prior to filtering) distribution 
plots in the HTML document and as a separate GD::Graph line plot.

- Pipeline produces an HTML report when following the double-digest 
protocol.

- HTML file reports percentage unique di-tags following de-duplication.

- HiCUP version number and pipeline parameters are printed in the 
headers of the SAM/BAM files.

- User can specify hi-c ligation sequences directly via hicup.conf

- Fixed bug causing HTML reports not to be written if a single sample 
did not pass completely through the pipeline.

- Fixed bug preventing 'longest:' or 'shortest:' config option being 
left blank in hicup.conf


RELEASE NOTES FOR HICUP v0.4.1 (23 September 2013)
--------------------------------------------------
HiCUP v0.4.1 is a minor update incorporating the changes described below:

- Fixed bug causing results from different datasets to become mixed in
the HTML report.

- Improved HTML report layout.


RELEASE NOTES FOR HICUP v0.4.0 (10 September 2013)
--------------------------------------------------
HiCUP v0.4.0 is a major update incorporating the changes described below:

- A di-tag is defined by each of its constituent reads and each read is 
now defined by 1) the position in the reference genome to which the first 
base of the sequence read (putative sonication cut site) map; and 2) the 
orientation of the sequence read with respect to the reference genome. 
The read end position is no longer considered.

- Fixed bug in hicup_deduplicator preventing it identifying all 
duplicates if the sequencing run was performed with a different number 
of cycles. 

- Added --nofill option to hicup_truncater (and consequently hicup) 
allowing users to specify that a fill-in of sticky ends was not 
performed. 

- Made slight adjustment to hicup_truncator to prevent division by zero 
errors when working out the %truncation rate.

- hicup_truncater now truncates upstream of the restriction enzyme 
recognition site, not at the restriction enzyme cut site.

- hicup_mapper now reports the number of reads that were too short to 
be sent to Bowtie. The mapping efficiency will refer to: 
(100 * unique alignments) / 
(reads too short to send to Bowtie + reads sent to Bowtie).

- hicup_filter now discriminates between same-fragment dangling ends 
and same-fragment internal. 

- HiCUP master script edited so the file processes all the reads in a 
file to determine FASTQ quality scores.

- Added a check in the pipeline to confirm the number of forward and 
reverse reads are the same in the FASTQ file pairs (some users appear 
to filter reads in some way prior passing to HiCUP).

- Users can now specify an output directory to which output files are 
written.

- All summary files now display the same datestamp.

- HiCUP sonication protocol now generates an HTML report summarising the 
results of each file processed.


RELEASE NOTES FOR HICUP v0.3.0 (02 November 2012)
-------------------------------------------------
HiCUP v0.3.0 is a major update incorporating the changes described below:

- hicup_sorter removed from the pipeline. Users should now de-multiplex 
samples using other software.

- The pipeline determines automatically the FASTQ format (e.g. Sanger) 
adopted if this was not specified by the user.  It may not always be 
possible to determine this unambiguously and in such circumstances the 
pipeline selects the most likely option.

- The pipeline will try to determine the path to Bowtie if this is not 
specified by the user. Also, fixed a bug in how HiCUP identifies the 
location of SAMtools.

- Improved how the pipeline checks Bowtie indices have been specified 
correctly by the user.


RELEASE NOTES FOR HICUP v0.2.2 (03 August 2012)
-----------------------------------------------
HiCUP v0.2.2 is a minor update adding the functionality described below:

- The mapping process is now less memory intensive, with Bowtie 
option --chunkmbs lowered from 1024 to 512. Also, reads shorter than
20bps are no longer passed to Bowtie.

- Fixed bug preventing HiCUP processing files in a separate folder from 
the hicup.conf configuration file.

- The hicup master script terminates immediately if another pipeline 
script dies. 


RELEASE NOTES FOR HICUP v0.2.1 (19 July 2012)
---------------------------------------------
HiCUP v0.2.1 is a minor update adding the functionality described below:

- The hicup_filter script now calculates the number of di-tags whose
alignment to the reference genome suggests they were created by a single 
restriction fragment circularizing and ligating to itself, and then 
following sonication forms a liner molecule containing the modified Hi-C 
restriction site. These di-tags were previously classified as 
'Same Fragment' di-tags, but this category now comprises all di-tags 
in which both reads map to the same restriction fragment except those 
classified as derived from circularized molecules.

- The hicup_filter summary file now reports the number of di-tags 
falling within each category, not the percentage summary. 


RELEASE NOTES FOR HICUP v0.2.0 (26 June 2012)
---------------------------------------------
HiCUP v0.2.0 is a major update providing extra functionality in addition 
to the removal of several bugs. The most significant changes are 
described below:

- Addition of a new script ('hicup_deduplicator') at the end of the 
pipeline for removing duplicate di-tags (most probably PCR artefacts). 
The script generates a text file summarising the results, along with a 
pie chart (*.png format) of the cis/trans ratio of di-tags present in 
the final dataset.

- hicup_mapper and hicup_pairer combined into a single script called 
'hicup_mapper'. In addition to reporting Bowtie mapping efficiencies, 
the script also reports the percentage read pairs in which both 
forward and reverse reads could be aligned to the reference genome.

- hicup_filter, when processing Hi-C data generated using the Hi-C 
sonication protocol, now rejects di-tags on the basis of size AFTER 
all other filters have been passed.

- hicup_filter produces a less verbose summary file and produces pie 
charts of the results (*.png format).

- hicup_filter modified so when following the sonication protocol it 
identifies and rejects di-tags containing re-ligated fragments, not 
simply those on adjacent fragments. It does this by checking that the 
fragments are adjacent (as before) and in the same orientation - which 
means the forward and reverse reads will be on opposite strands 
(+ and -).

- hicup_truncater now reports the average length of a truncated 
sequence.

- Fixed a bug causing hicup_digester to only process the last 
chromosome in a file containing multiple chromosomes.


RELEASE NOTES FOR HICUP v0.1.1
------------------------------

HiCUP v0.1.1 is an alpha release and as such is still a work in progress.

Although the core functionality of the program should all now be 
present, it is very much still a work in progress and you should bear 
this mind when running the software.