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AGEseq.conf
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#AGEseq2.0
#Change parameters for Ageseq reporting accordingly
mismatch_cutoff = 0.1 ; # mismatch rate to filter low quality alignment, default 0.1 (10 %)
min_cutoff = 0 ; # cutoff to filter reads with low abundance, default 0
wt_like_report = 20 ; # report top xx WT like records, default 20
indel_report = 50 ; # report top xx records with indel, default 50
## Above parameters from v1 are abandonded in current version
remove_files = 1 ; # keep (0) or delete (1) intermediate files, default = 1
READ_SNP_MINR_FREQ = 0.05 ;
READ_INDEL_MINR_FREQ = 0.001 ;
READ_SNP_MINIMAL_SUPPORT = 3 ;
READ_INDEL_MINIMAL_SUPPORT = 3 ;
WOBBLE_BASE = True ; #Treat wobble base as one allele?
WOBBLE_FREQ_LOW = 0.35 ; #Minimal frequency to call a wobble base
WOBBLE_FREQ_HIGH = 0.75 ; #Maximum frequency to call a wobble base
#Below are paramters for BLAT
blat_tileSize = 7 ;
blat_oneOff = 1 ;
blat_maxGap = 20 ;
blat_minIdentity = 70 ;
blat_minScore = 80 ;