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field_of_view.html
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<!-- comment -->
<!DOCTYPE html>
<html lang="en">
<head>
<meta charset="utf-8">
<meta name="viewport" content="width=device-width, initial-scale=1.0">
<link rel="stylesheet" href="stylesheets/style.css">
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<title>amsikking: Field of view</title>
</head>
<body>
<header>
<nav>
<ul>
<li><h1 style="margin-top: 0rem; margin-left: 1rem; margin-right: 1rem">amsikking:</h1></li>
<li><a href="https://amsikking.github.io/">Homepage</a></li>
<li><a href="https://github.com/amsikking/microscope_objectives">GitHub</a></li>
</ul>
</nav>
</header>
<main>
<h1>Microscope objectives</h1>
<p>An introduction to 'infinity' corrected microscope objectives.</p>
<a href="./index.html">Contents</a>
<section>
<h2>Field of view</h2>
<p>
The field of view (\(FOV\)) of an objective is rarely specified, perhaps because
the definition is not standardised. For example, one could say that the \(FOV\)
is the range over which the image is diffraction limited at the maximum \(NA\)
(a stringent definition giving the smallest fields, but arguably the most correct
choice). Alternatively one could accept the field over which stigmatic imaging
is maintained for some fraction of the maximum \(NA\) (for example 80%). In
reality, without full design information for the objective and tube lens,
<strong>we simply don't know the field of view</strong>. It is common
to <em>estimate</em> the \(FOV\) using the tube lens field number \(FN\) and
objective magnification \(M_T\):
\[ FOV \sim \frac{FN}{M_T} \tag{1}\]
</p>
<figure>
<img src="figures/field_of_view.png" alt="field_of_view.png">
<figcaption>
(<a href="figures/objective_sketches.odp">.odp sketch</a>)
</figcaption>
</figure>
</section>
</main>
</body>
</html>