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working_distance.html
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<!DOCTYPE html>
<html lang="en">
<head>
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<title>amsikking: Working distance</title>
</head>
<body>
<header>
<nav>
<ul>
<li><h1 style="margin-top: 0rem; margin-left: 1rem; margin-right: 1rem">amsikking:</h1></li>
<li><a href="https://amsikking.github.io/">Homepage</a></li>
<li><a href="https://github.com/amsikking/microscope_objectives">GitHub</a></li>
</ul>
</nav>
</header>
<main>
<h1>Microscope objectives</h1>
<p>An introduction to 'infinity' corrected microscope objectives.</p>
<a href="./index.html">Contents</a>
<section>
<h2>Working distance</h2>
<p>
The working distance (w.d.) is the range over which an objective
can be moved towards the infinity corrected image plane before it
mechanically collides with the sample
(<a class="citation" href="https://doi.org/10.1007/978-0-387-45524-2"
title="Handbook of Biological Confocal Microscopy, third edition;
J. Pawley; p157 , Springer US, ISBN 978-0-387-25921-5,
eBook ISBN 978-0-387-45524-2, (2006)">Pawley 2006</a>). So
importantly it is the <em>maximum depth of standard focus</em>
(where the objective is moved towards the sample or vice
versa). For a typical air objective this can simply be the
distance from the front surface of the objective to the image
plane:
</p>
<figure>
<img src="figures/working_distance.png" alt="working_distance.png">
<figcaption>
(<a href="figures/objective_sketches.odp">.odp sketch</a>)
</figcaption>
</figure>
<p>
For a <em>coverslip</em> corrected objective it is the
distance to the coverslip when the image plane is
<em>immediately above</em> the coverslip:
</p>
<figure>
<img src="figures/working_distance_coverslip.png" alt="working_distance_coverslip.png">
<figcaption>
(<a href="figures/objective_sketches.odp">.odp sketch</a>)
</figcaption>
</figure>
</section>
</main>
</body>
</html>