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config.yml
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# Location of PopPIPE repository relative to the Snakefile
poppipe_location: .
# Files output by the PopPUNK run
poppunk_rfile: rfiles.txt
poppunk_clusters: database_clusters.csv
poppunk_h5: database.h5
transmission_metadata: transmission_metadata.csv
# Minimum cluster size to subcluster
min_cluster_size: 6
# ska options
ska:
fastq_qual: 20
fastq_cov: 4
kmer: 31
single_strand: false
freq_filter: 0.9
# IQ-TREE options
iqtree:
enabled: true
mode: full
model: 012310+G+ASC # +ASC recommended. May replace 012310 with GTR for single strand data
# fastbaps options
fastbaps:
# Number of hierarchical clustering levels
levels: 2
# mandrake options
mandrake:
knn: 50
perplexity: 25
maxIter: 100000
# Microreact title, and your contact details
microreact:
name: Listeria PopPIPE
website: https://www.poppunk.net
email: [email protected]
api_token: a1b2c3d4 ## see https://docs.microreact.org/api/access-tokens
# gubbins options
gubbins:
prefix: gubbins
tree_builder: iqtree
min_snps: 3
min_window_size: 100
max_window_size: 10000
iterations: 5
# transphylo options
transphylo:
w_shape: 10
w_scale: 0.1
mcmcIterations: 1000
startNeg: 0.1
startOff_r: 1
startOff_p: 0.5
startPi: 0.5
optiStart: 2
dateT: 0