diff --git a/vault/open-notebook.commons.rouchene.2024.11.07.md b/vault/open-notebook.commons.rouchene.2024.11.07.md
index 270a737b04..8bb7da2c7b 100644
--- a/vault/open-notebook.commons.rouchene.2024.11.07.md
+++ b/vault/open-notebook.commons.rouchene.2024.11.07.md
@@ -2,7 +2,7 @@
 id: v53lfrwq8py2ghpieoz4zb8
 title: '2024-11-07'
 desc: ''
-updated: 1730978825173
+updated: 1730980042950
 created: 1730978776613
 traitIds:
   - open-notebook-commons-rouchene
@@ -15,33 +15,42 @@ traitIds:
 Today is 2024.11.07
 
 ## Todo today
+PCR and purification of V and CH44S DNA 
 
 ### Have a look at the COMMONS research discussion forum
     - https://github.com/orgs/commons-research/discussions
 
-### Daily work on the Microcosmics project
+### Daily work on the Microcosmics project 
 
 See [[projects.commons-public.microcosmics]]
 
 
 ###
+
+
 ###
 
 ## Doing
+DNA purification with kit this afternoon.
 
 ## Paused
 
 ## Done
+PCR and gel migration.
 
 ## Notes
+i get beautiful band for CH4 and bad one of V1. 
 
 ## Todo tomorrow, one day ... or never 
 
 
+
 ###
 ###
 
 
 ## Today I learned that
+Even though I have good DNA, it doesn't mean that sequencing is guaranteed. 
+If the sequencing doesn't work with the forward primer, we can try the reverse primer, if our DNA is well amplified. 
 
 - By doing ctrl+o ctrl+n I can open a daily note 
\ No newline at end of file
diff --git a/vault/open-notebook.commons.rouchene.2024.11.08.md b/vault/open-notebook.commons.rouchene.2024.11.08.md
new file mode 100644
index 0000000000..4af247d1ef
--- /dev/null
+++ b/vault/open-notebook.commons.rouchene.2024.11.08.md
@@ -0,0 +1,52 @@
+---
+id: pumadz557ero8tthitm86eq
+title: '2024-11-08'
+desc: ''
+updated: 1731066925969
+created: 1731054899844
+traitIds:
+  - open-notebook-commons-rouchene
+---
+
+
+
+# This is Rimas's COMMONS Lab daily Open Notebook.
+
+Today is 2024.11.08
+
+## Todo today
+Agarose gel for V1 DNA extracted with the kit. I want to decrease the migration time to 20 minutes to observe the results.
+
+### Have a look at the COMMONS research discussion forum
+    - https://github.com/orgs/commons-research/discussions
+
+### Daily work on the Microcosmics project
+
+See [[projects.commons-public.microcosmics]]
+
+
+###
+### for DNA identification i need 
+ladder 1kb for bacteria and 800 pb for fungi.
+primers 27F and 1492R 
+Solifast 
+
+
+## Doing
+
+## Paused
+
+## Done
+
+## Notes
+
+## Todo tomorrow, one day ... or never 
+
+
+###
+###
+
+
+## Today I learned that
+
+- 
\ No newline at end of file
diff --git a/vault/open-notebook.commons.rouchene.2024.11.11.md b/vault/open-notebook.commons.rouchene.2024.11.11.md
new file mode 100644
index 0000000000..3fc45cffd5
--- /dev/null
+++ b/vault/open-notebook.commons.rouchene.2024.11.11.md
@@ -0,0 +1,51 @@
+---
+id: 07fv2mkig1ep34hxpt0ocer
+title: '2024-11-11'
+desc: ''
+updated: 1731336849066
+created: 1731324134768
+traitIds:
+  - open-notebook-commons-rouchene
+---
+
+
+
+# This is Rimas's COMMONS Lab daily Open Notebook.
+
+Today is 2024.11.11
+
+## Todo today
+Putification of V1 DNA.
+Preparation of PDB.
+Information on CH4's pathogenicity.
+### Have a look at the COMMONS research discussion forum
+    - https://github.com/orgs/commons-research/discussions
+
+### Daily work on the Microcosmics project
+
+See [[projects.commons-public.microcosmics]]
+
+
+###
+###
+
+## Doing
+Litterature on my species! 
+## Paused
+
+## Done
+
+## Notes
+
+## Todo tomorrow, one day ... or never 
+complet table of strains.
+Litterature on my species!  
+
+
+###
+###
+
+
+## Today I learned that
+
+- 
\ No newline at end of file