diff --git a/README.md b/README.md
index 485c0404..a5e899b3 100644
--- a/README.md
+++ b/README.md
@@ -319,6 +319,7 @@ provider, such as `ts-node`. Otherwise, widening the acceptance extension here w
   ```
 
 ## Override TypeScript detection using an environment variable
+This plugin uses [native type stripping](https://nodejs.org/docs/latest-v23.x/api/typescript.html#modules-typescript) with Node 23 and later.
 
 It is possible to override the automatic detection of a TypeScript-capable runtime using the `FASTIFY_AUTOLOAD_TYPESCRIPT` environment variable. If set to a truthy value Autoload will load `.ts` files, expecting that node has a TypeScript-capable loader.
 
diff --git a/lib/find-plugins.js b/lib/find-plugins.js
index baffd7af..939f4c99 100644
--- a/lib/find-plugins.js
+++ b/lib/find-plugins.js
@@ -144,9 +144,16 @@ function accumulatePlugin ({ file, type, opts, pluginTree, prefix }) {
 }
 
 function handleTypeScriptSupport (file, language, isHook = false) {
-  if (language === 'typescript' && !runtime.supportTypeScript) {
-    throw new Error(`@fastify/autoload cannot import ${isHook ? 'hooks ' : ''}plugin at '${file}'. To fix this error compile TypeScript to JavaScript or use 'ts-node' to run your app.`)
-  }
+  if (
+    language === 'typescript' &&
+    /* c8 ignore start - This cannot be reached from Node 23 native type-stripping */
+    !runtime.supportTypeScript
+  ) {
+    throw new Error(
+      `@fastify/autoload cannot import ${isHook ? 'hooks ' : ''}plugin at '${file}'. To fix this error compile TypeScript to JavaScript or use 'ts-node' to run your app.`
+    )
+  }
+  /* c8 ignore end */
 }
 
 function filterPath (path, filter) {
diff --git a/lib/runtime.js b/lib/runtime.js
index 0dd3cd5a..11182407 100644
--- a/lib/runtime.js
+++ b/lib/runtime.js
@@ -116,7 +116,8 @@ Object.defineProperties(runtime, {
         runtime.tsm ||
         runtime.tsx ||
         runtime.esbuild ||
-        runtime.tsimp
+        runtime.tsimp ||
+        runtime.nodeVersion >= 23
       )
       return cache.supportTypeScript
     }
@@ -130,7 +131,13 @@ Object.defineProperties(runtime, {
       )
       return cache.forceESM
     }
-  }
+  },
+  nodeVersion: {
+    get () {
+      cache.nodeVersion ??= Number(process.version.split('.')[0].slice(1))
+      return cache.nodeVersion
+    },
+  },
 })
 
 module.exports = runtime
diff --git a/package.json b/package.json
index 60ff2270..831731ac 100644
--- a/package.json
+++ b/package.json
@@ -8,7 +8,7 @@
   "scripts": {
     "lint": "eslint",
     "lint:fix": "eslint --fix",
-    "test": "npm run typescript && npm run typescript:jest && npm run typescript:swc-node-register && npm run typescript:tsm && npm run typescript:tsx && npm run typescript:vitest && npm run typescript:esbuild && npm run unit",
+    "test": "npm run typescript && npm run typescript:native && npm run typescript:jest && npm run typescript:swc-node-register && npm run typescript:tsm && npm run typescript:tsx && npm run typescript:vitest && npm run typescript:esbuild && npm run unit",
     "typescript": "tsd",
     "typescript:jest": "jest",
     "typescript:esm": "node scripts/unit-typescript-esm.js",
@@ -17,6 +17,7 @@
     "typescript:tsx": "node scripts/unit-typescript-tsx.js",
     "typescript:tsimp": "node scripts/unit-typescript-tsimp.js",
     "typescript:esbuild": "node scripts/unit-typescript-esbuild.js",
+    "typescript:native": "node scripts/unit-typescript-native-type-stripping.js",
     "typescript:vitest": "vitest run",
     "typescript:vitest:dev": "vitest",
     "unit": "node scripts/unit.js",
diff --git a/scripts/unit-typescript-native-type-stripping.js b/scripts/unit-typescript-native-type-stripping.js
new file mode 100644
index 00000000..6e57a543
--- /dev/null
+++ b/scripts/unit-typescript-native-type-stripping.js
@@ -0,0 +1,30 @@
+'use strict'
+
+const { exec } = require('node:child_process')
+const runtime = require('../lib/runtime')
+
+function common () {
+  const args = ['node', 'test/typescript-common/index.ts']
+  const child = exec(args.join(' '), {
+    shell: true,
+  })
+  child.stdout.pipe(process.stdout)
+  child.stderr.pipe(process.stderr)
+  child.once('close', esm)
+}
+
+function esm () {
+  const args = ['node', 'test/typescript-esm/forceESM.ts']
+
+  const child = exec(args.join(' '), {
+    shell: true,
+  })
+
+  child.stdout.pipe(process.stdout)
+  child.stderr.pipe(process.stderr)
+  child.once('close', process.exit)
+}
+
+if (runtime.nodeVersion >= 23) {
+  common()
+}
diff --git a/scripts/unit-typescript-tsx.js b/scripts/unit-typescript-tsx.js
index d76db821..d14f406f 100644
--- a/scripts/unit-typescript-tsx.js
+++ b/scripts/unit-typescript-tsx.js
@@ -1,12 +1,11 @@
 'use strict'
 
 const { exec } = require('node:child_process')
-
-const version = Number(process.version.split('.')[0].slice(1))
+const runtime = require('../lib/runtime')
 
 const args = [
   'npx',
-  version >= 18 ? '--node-options=--import=tsx' : '',
+  runtime.nodeVersion >= 18 ? '--node-options=--import=tsx' : '',
   'tsnd',
   'test/typescript/basic.ts'
 ]
diff --git a/test/commonjs/error.js b/test/commonjs/error.js
index d961bf6c..7070003a 100644
--- a/test/commonjs/error.js
+++ b/test/commonjs/error.js
@@ -2,9 +2,12 @@
 
 const t = require('tap')
 const fastify = require('fastify')
+const runtime = require('../../lib/runtime')
+
+const typeStrippingEnabled = runtime.nodeVersion >= 23
 
 t.test('independent of module support', function (t) {
-  t.plan(8)
+  t.plan(typeStrippingEnabled ? 7 : 8)
   const app = fastify()
 
   app.register(require('./syntax-error/app'))
@@ -28,8 +31,12 @@ t.test('independent of module support', function (t) {
   app3.register(require('./ts-error/app'))
 
   app3.ready(function (err) {
-    t.type(err, Error)
-    t.match(err.message, /cannot import plugin.*typescript/i)
+    if (typeStrippingEnabled) {
+      t.error(err)
+    } else {
+      t.type(err, Error)
+      t.match(err.message, /cannot import plugin.*typescript/i)
+    }
   })
 
   const app4 = fastify()
diff --git a/test/issues/369/test.js b/test/issues/369/test.js
index 025f11bc..5bc9cfa7 100644
--- a/test/issues/369/test.js
+++ b/test/issues/369/test.js
@@ -4,6 +4,7 @@ const { test } = require('tap')
 const Fastify = require('fastify')
 const path = require('node:path')
 const autoload = require('../../..')
+const runtime = require('../../../lib/runtime')
 
 test('Should throw an error when trying to load invalid hooks', async (t) => {
   const app = Fastify()
@@ -22,7 +23,11 @@ test('Should throw an error when trying to import hooks plugin using index.ts if
     autoHooks: true
   })
 
-  await t.rejects(app.ready(), new Error(`@fastify/autoload cannot import hooks plugin at '${path.join(__dirname, 'invalid-index-type/index.ts')}'`))
+  if (runtime.nodeVersion >= 23) {
+    t.doesNotThrow(() => app.ready())
+  } else {
+    await t.rejects(app.ready(), new Error(`@fastify/autoload cannot import hooks plugin at '${path.join(__dirname, 'invalid-index-type/index.ts')}'`))
+  }
 })
 
 test('Should not accumulate plugin if doesn\'t comply to matchFilter', async (t) => {
diff --git a/test/typescript-common/basic/app.ts b/test/typescript-common/basic/app.ts
new file mode 100644
index 00000000..4b228bdf
--- /dev/null
+++ b/test/typescript-common/basic/app.ts
@@ -0,0 +1,12 @@
+import type { FastifyInstance } from 'fastify'
+
+const { join } = require('node:path')
+const fastifyAutoLoad = require('../../../index.js')
+
+module.exports = function (fastify: FastifyInstance, _opts: any, next: any) {
+  fastify.register(fastifyAutoLoad, {
+    dir: join(__dirname, 'foo'),
+  })
+
+  next()
+}
diff --git a/test/typescript-common/basic/foo/javascript.js b/test/typescript-common/basic/foo/javascript.js
new file mode 100644
index 00000000..78bbcf63
--- /dev/null
+++ b/test/typescript-common/basic/foo/javascript.js
@@ -0,0 +1,9 @@
+'use strict'
+
+module.exports = function (fastify, _opts, next) {
+  fastify.get('/javascript', (_request, reply) => {
+    reply.send({ script: 'java' })
+  })
+
+  next()
+}
diff --git a/test/typescript-common/basic/foo/shouldBeIgnored.d.ts b/test/typescript-common/basic/foo/shouldBeIgnored.d.ts
new file mode 100644
index 00000000..16f1a352
--- /dev/null
+++ b/test/typescript-common/basic/foo/shouldBeIgnored.d.ts
@@ -0,0 +1,3 @@
+// See https://github.com/fastify/fastify-autoload/issues/65
+declare const definitionFilesAreIncluded: never
+export default definitionFilesAreIncluded
diff --git a/test/typescript-common/basic/foo/typescript.ts b/test/typescript-common/basic/foo/typescript.ts
new file mode 100644
index 00000000..97cc2c3c
--- /dev/null
+++ b/test/typescript-common/basic/foo/typescript.ts
@@ -0,0 +1,7 @@
+module.exports = function (fastify: any, _opts, next) {
+  fastify.get('/typescript', (_request, reply) => {
+    reply.send({ script: 'type' })
+  })
+
+  next()
+}
diff --git a/test/typescript-common/index.ts b/test/typescript-common/index.ts
new file mode 100644
index 00000000..33c81b7d
--- /dev/null
+++ b/test/typescript-common/index.ts
@@ -0,0 +1,40 @@
+'use script'
+
+const t = require('tap')
+const fastify = require('fastify')
+
+const basicApp = require('./basic/app.ts')
+
+t.plan(5)
+
+const app = fastify()
+
+app.register(basicApp)
+
+app.ready(async function (err) {
+  t.error(err)
+
+  await app
+    .inject({
+      url: '/javascript',
+    })
+    .then(function (res: any) {
+      t.equal(res.statusCode, 200)
+      t.same(JSON.parse(res.payload), { script: 'java' })
+    })
+    .catch((err) => {
+      t.error(err)
+    })
+
+  await app
+    .inject({
+      url: '/typescript',
+    })
+    .then(function (res: any) {
+      t.equal(res.statusCode, 200)
+      t.same(JSON.parse(res.payload), { script: 'type' })
+    })
+    .catch((err) => {
+      t.error(err)
+    })
+})
diff --git a/test/typescript-esm/app/index.ts b/test/typescript-esm/app/index.ts
index fe08fa2a..dfe4f07a 100644
--- a/test/typescript-esm/app/index.ts
+++ b/test/typescript-esm/app/index.ts
@@ -1,4 +1,4 @@
-import { FastifyInstance, FastifyReply, FastifyRequest } from 'fastify'
+import type { FastifyInstance, FastifyReply, FastifyRequest } from 'fastify'
 
 export default function (fastify: FastifyInstance, _: object, next: (err?: Error) => void): void {
   fastify.get('/installed', (_: FastifyRequest, reply: FastifyReply): void => {
diff --git a/test/typescript-esm/forceESM.ts b/test/typescript-esm/forceESM.ts
index 64111034..be9bb771 100644
--- a/test/typescript-esm/forceESM.ts
+++ b/test/typescript-esm/forceESM.ts
@@ -2,7 +2,7 @@ import fastify from 'fastify'
 import { dirname, resolve } from 'node:path'
 import { fileURLToPath } from 'node:url'
 import t from 'tap'
-import fastifyAutoLoad from '../../'
+import fastifyAutoLoad from '../../index.js'
 
 t.plan(4)