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Rhea mapping GO:0010308 | acireductone dioxygenase (Ni2+-requiring) activity #5616

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ValWood opened this issue Jan 22, 2025 · 1 comment

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@ValWood
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ValWood commented Jan 22, 2025

GO:0010308 | acireductone dioxygenase (Ni2+-requiring) activity | IEA with 14161 | GO_REF:0000116

can you confirm that this is present in yeast (or higher eukaryotes)? (Produces carbon monoxide)

Most of the evidence I can find is that the yeast enzyme is
GO:0010309 | acireductone dioxygenase [iron(II)-requiring] activity

and most of the evidence on the Rhea page supports this:
https://www.rhea-db.org/rhea/14161

@ValWood
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ValWood commented Jan 22, 2025

problem annotations
https://www.pombase.org/gene/SPBC887.01

SGD has annotation to
GO:0010308 | acireductone dioxygenase (Ni2+-requiring) activity by IMP but this might be misannotation.

Particularly since the human UniProtKB entry has a comment
Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4-methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway (PubMed:15938715).
Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway (By similarity).

It is possible that both activities exist, and that acireductone dioxygenase (Ni2+-requiring) is involved in an alternative pathway, but I haven't been able to locate any evidence for this.

@amorgat could you advise?
Many thanks
Val

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