diff --git a/docs/pipelines.md b/docs/pipelines.md index c45c90c..62b888e 100644 --- a/docs/pipelines.md +++ b/docs/pipelines.md @@ -2,6 +2,35 @@ Content - ![](https://img.shields.io/badge/status-WorkInProgress-yellow) +## Paramters + +### RNAseq + +- we use salmon with bam files mapped to transcriptome for quantification + +### CHIPseq + +- defaults parameters +- de-duplication for all samples +- bowtie is set up with these extra parameters: `--sensitive-local -X 1000` +- macs_gsize needs to be setup for each species accordingly ... tools + +### CUT&RUN + +- defaults parameters +- turn on dedup_target_reads + +### ATACseq + +All peaks `nf-core-atac-seq_shift`: +- shift is on +- keep_dup is false + +NFR peaks `nf-core-atac-seq_shift_NFR`: +- same than previous except parameters for Aligmentsieve: + - `--minFragmentLength 0` + - `--maxFragmentLength 120` + ## Nextflow in Seqera platform - Create an user here: https://cloud.seqera.io/login