issue with "ord <- hclust(dist(t(p$pan_matrix[, names(hload) ])))$order"

[sushanta26]

Hi Thank you for this awesome R program

I am facing problem at Plotting heatmap associated with a tree, I am following example r script Cfetus_pangenome_example.R in my data set , input file was designed as given example dataset.

ord <- hclust(dist(t(p$pan_matrix[, names(hload) ])))$order

Error in hclust(dist(t(p$pan_matrix[, names(hload)]))) :
must have n >= 2 objects to cluster

I am not sure where I am making mistake , previous command were running perfect this step showing error

running this "print(p$pan_matrix[, names(hload)]) " to check data structure has given me following output
Staphylococcus_capitis_C87
2
Staphylococcus_capitis_CCM_2734
4
Staphylococcus_capitis_CR01
3
Staphylococcus_capitis_CR01_strain_DE0445
0
Staphylococcus_capitis_CR02
0
Staphylococcus_capitis_CR03
3
Staphylococcus_capitis_CR04
2
Staphylococcus_capitis_CR05
1
Staphylococcus_capitis_CR09
1
Staphylococcus_capitis_QN1
1
Staphylococcus_capitis_SC32
0
Staphylococcus_capitis_SC35
0
Staphylococcus_capitis_SK14
1
Staphylococcus_capitis_TW2795

Need your help to understand where I am making mistake.

Thank you
Sushanta