CalExp2ddCt not recognizing groups with varied numbers of BioRep

[maqy15]

Hi! I'm trying to analyze my RT-qPCR data (with 4 technical replicates) using the 2^(-ddCt) method.

While I was trying to exclude some very obvious Cq outliers, I noticed that the CalExp2ddCt function does not recognize an input data table that contains groups with varied BioRep numbers properly. Is there a way to strip the outliers from the results? Or is there a way to remove them from the input datasheet without disturbing the output?

Thanks a lot!

[lixiang117423]

I will fix that as soon as possible. I've been busy writing my graduation thesis recently.

[lixiang117423]

we have added remove.outliers parameter, could you please try it again, pease let me know it works or not.

[roywhite98]

详哥，为啥这个包的ref.group的2-ddct不是1，看您文章的图也有这个问题，很困惑，望指教！