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Screen guides to ensure they do not contain cut sites for restriction enzymes #7

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lzamparo opened this issue Dec 12, 2017 · 1 comment
Open

Screen guides to ensure they do not contain cut sites for restriction enzymes #7

lzamparo opened this issue Dec 12, 2017 · 1 comment
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@lzamparo
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These enzymes will be used for cloning either target into the contsruct for mouse genome wide screens, or the reporter:

BstX1
Blp1
BamH1
@lzamparo
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No guide can contain these sequences, nor can a guide contain a subsequence which extends beyond the guide into a match pursuant in the adjacent part of the construct

@lzamparo lzamparo added the TODO label Dec 12, 2017
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