diff --git a/.github/workflows/wave.yml b/.github/workflows/wave.yml
new file mode 100644
index 00000000000..849e59ee2b2
--- /dev/null
+++ b/.github/workflows/wave.yml
@@ -0,0 +1,87 @@
+name: Generate conda-lock
+# When environment.yml is changed
+on:
+  pull_request:
+    paths:
+      - "**/environment.yml"
+
+jobs:
+  gen-matrix:
+    name: generate-matrix
+    runs-on: ubuntu-latest
+
+    steps:
+      - uses: actions/checkout@a5ac7e51b41094c92402da3b24376905380afc29 # v4
+
+      - name: Calculate file differences
+        id: diff
+        uses: tj-actions/changed-files@v44
+        with:
+          json: true
+          quotepath: false
+          files: |
+            modules/**/environment.yml
+      - name: Debug
+        run: echo ${{ steps.diff.outputs.all_changed_files }}
+      - id: set-matrix
+        run: echo "matrix={\"files\":${{ steps.diff.outputs.all_changed_files }} }" >> "$GITHUB_OUTPUT"
+
+    outputs:
+      matrix: ${{ steps.set-matrix.outputs.matrix }}
+
+  lock:
+    if: "${{ fromJson(needs.gen-matrix.outputs.matrix) }}"
+    needs: gen-matrix
+    name: Generate conda-lock file
+    permissions:
+      contents: write
+    runs-on: ubuntu-latest
+    strategy:
+      fail-fast: false
+      matrix: "${{ fromJson(needs.gen-matrix.outputs.matrix) }}"
+    steps:
+      - uses: actions/checkout@a5ac7e51b41094c92402da3b24376905380afc29 # v4
+        with:
+          ref: ${{ github.event.pull_request.head.ref }}
+
+      - uses: mamba-org/setup-micromamba@v1
+        with:
+          environment-name: conda-lock-env
+          create-args: >-
+            python=3.11
+            conda-lock
+            mamba
+
+      - name: Get the lock file path
+        uses: actions/github-script@v7
+        id: lockfile-name
+        with:
+          result-encoding: string
+          script: |
+            return '${{ matrix.files }}'.replace('/environment.yml', '/conda-lock.yml');
+
+      - name: Run conda-lock
+        run: |
+          rm --force "${{steps.lockfile-name.outputs.result}}"
+          conda-lock lock $MAMBA --file "${{ matrix.files }}" --kind lock --platform linux-64 --lockfile "${{steps.lockfile-name.outputs.result}}"
+        env:
+          MAMBA: "--mamba"
+        shell: bash -l {0}
+
+      - name: Commit & push changes
+        id: commit-and-push
+        run: |
+          git config user.email "core@nf-co.re"
+          git config user.name "nf-core-bot"
+          git config push.default upstream
+          git add .
+          git status
+          git commit -m "[automated] autogenerated conda-lock file"
+          git push
+
+      - name: If no conda-lock update
+        if: steps.commit-and-push.outcome == 'failure'
+        run: |
+          echo "No conda-lock update"
+
+        # TODO Build containers with Wave
diff --git a/.prettierignore b/.prettierignore
index 41fc3479697..cb3f3e47fb4 100644
--- a/.prettierignore
+++ b/.prettierignore
@@ -15,3 +15,4 @@ __pycache__
 *.pyo
 *.pyc
 .github/renovate.json5
+**/conda-lock.yml
diff --git a/modules/nf-core/bowtie2/align/conda-lock.yml b/modules/nf-core/bowtie2/align/conda-lock.yml
new file mode 100644
index 00000000000..b3725d07f66
--- /dev/null
+++ b/modules/nf-core/bowtie2/align/conda-lock.yml
@@ -0,0 +1,615 @@
+# This lock file was generated by conda-lock (https://github.com/conda/conda-lock). DO NOT EDIT!
+#
+# A "lock file" contains a concrete list of package versions (with checksums) to be installed. Unlike
+# e.g. `conda env create`, the resulting environment will not change as new package versions become
+# available, unless you explicitly update the lock file.
+#
+# Install this environment as "YOURENV" with:
+#     conda-lock install -n YOURENV conda-lock.yml
+# To update a single package to the latest version compatible with the version constraints in the source:
+#     conda-lock lock  --lockfile conda-lock.yml --update PACKAGE
+# To re-solve the entire environment, e.g. after changing a version constraint in the source file:
+#     conda-lock -f environment.yml --lockfile conda-lock.yml
+version: 1
+metadata:
+  content_hash:
+    linux-64: b864a48e350c8ce3992e145bfed6961e07ccf9dc5a7e67b0a03943d2f2d87e94
+  channels:
+  - url: conda-forge
+    used_env_vars: []
+  - url: bioconda
+    used_env_vars: []
+  - url: defaults
+    used_env_vars: []
+  platforms:
+  - linux-64
+  sources:
+  - environment.yml
+package:
+- name: _libgcc_mutex
+  version: '0.1'
+  manager: conda
+  platform: linux-64
+  dependencies: {}
+  url: https://conda.anaconda.org/conda-forge/linux-64/_libgcc_mutex-0.1-conda_forge.tar.bz2
+  hash:
+    md5: d7c89558ba9fa0495403155b64376d81
+    sha256: fe51de6107f9edc7aa4f786a70f4a883943bc9d39b3bb7307c04c41410990726
+  category: main
+  optional: false
+- name: _openmp_mutex
+  version: '4.5'
+  manager: conda
+  platform: linux-64
+  dependencies:
+    _libgcc_mutex: '0.1'
+    libgomp: '>=7.5.0'
+  url: https://conda.anaconda.org/conda-forge/linux-64/_openmp_mutex-4.5-2_gnu.tar.bz2
+  hash:
+    md5: 73aaf86a425cc6e73fcf236a5a46396d
+    sha256: fbe2c5e56a653bebb982eda4876a9178aedfc2b545f25d0ce9c4c0b508253d22
+  category: main
+  optional: false
+- name: bowtie2
+  version: 2.5.2
+  manager: conda
+  platform: linux-64
+  dependencies:
+    libgcc-ng: '>=12'
+    libstdcxx-ng: '>=12'
+    libzlib: '>=1.2.13,<1.3.0a0'
+    perl: ''
+    python: '>=3.10,<3.11.0a0'
+    python_abi: 3.10.*
+    tbb: '>=2021.10.0'
+    zlib: ''
+    zstd: '>=1.5.5,<1.6.0a0'
+  url: https://conda.anaconda.org/bioconda/linux-64/bowtie2-2.5.2-py310ha0a81b8_0.tar.bz2
+  hash:
+    md5: 81764fc4a9f32df1724741af3f82e93d
+    sha256: 39d1db19a8e6fdbdc4270d53511c3a93a06c69da87f78bc0e61e9d0159a25291
+  category: main
+  optional: false
+- name: bzip2
+  version: 1.0.8
+  manager: conda
+  platform: linux-64
+  dependencies:
+    __glibc: '>=2.17,<3.0.a0'
+    libgcc-ng: '>=12'
+  url: https://conda.anaconda.org/conda-forge/linux-64/bzip2-1.0.8-h4bc722e_7.conda
+  hash:
+    md5: 62ee74e96c5ebb0af99386de58cf9553
+    sha256: 5ced96500d945fb286c9c838e54fa759aa04a7129c59800f0846b4335cee770d
+  category: main
+  optional: false
+- name: c-ares
+  version: 1.32.3
+  manager: conda
+  platform: linux-64
+  dependencies:
+    __glibc: '>=2.17,<3.0.a0'
+    libgcc-ng: '>=12'
+  url: https://conda.anaconda.org/conda-forge/linux-64/c-ares-1.32.3-h4bc722e_0.conda
+  hash:
+    md5: 7624e34ee6baebfc80d67bac76cc9d9d
+    sha256: 3c5a844bb60b0d52d89c3f1bd828c9856417fe33a6102fd8bbd5c13c3351704a
+  category: main
+  optional: false
+- name: ca-certificates
+  version: 2024.7.4
+  manager: conda
+  platform: linux-64
+  dependencies: {}
+  url: https://conda.anaconda.org/conda-forge/linux-64/ca-certificates-2024.7.4-hbcca054_0.conda
+  hash:
+    md5: 23ab7665c5f63cfb9f1f6195256daac6
+    sha256: c1548a3235376f464f9931850b64b02492f379b2f2bb98bc786055329b080446
+  category: main
+  optional: false
+- name: htslib
+  version: '1.20'
+  manager: conda
+  platform: linux-64
+  dependencies:
+    bzip2: '>=1.0.8,<2.0a0'
+    libcurl: '>=8.8.0,<9.0a0'
+    libdeflate: '>=1.20,<1.22.0a0'
+    libgcc-ng: '>=12'
+    libzlib: '>=1.2.13,<2.0a0'
+    openssl: '>=3.3.1,<4.0a0'
+    xz: '>=5.2.6,<6.0a0'
+  url: https://conda.anaconda.org/bioconda/linux-64/htslib-1.20-h5efdd21_2.tar.bz2
+  hash:
+    md5: f0923cdb85b44dc77bdead8018645ea9
+    sha256: 791ef4ff30944a56803344b168ffb400b4a8d1ea52edb1c348541d2a67a838ba
+  category: main
+  optional: false
+- name: icu
+  version: '73.2'
+  manager: conda
+  platform: linux-64
+  dependencies:
+    libgcc-ng: '>=12'
+    libstdcxx-ng: '>=12'
+  url: https://conda.anaconda.org/conda-forge/linux-64/icu-73.2-h59595ed_0.conda
+  hash:
+    md5: cc47e1facc155f91abd89b11e48e72ff
+    sha256: e12fd90ef6601da2875ebc432452590bc82a893041473bc1c13ef29001a73ea8
+  category: main
+  optional: false
+- name: keyutils
+  version: 1.6.1
+  manager: conda
+  platform: linux-64
+  dependencies:
+    libgcc-ng: '>=10.3.0'
+  url: https://conda.anaconda.org/conda-forge/linux-64/keyutils-1.6.1-h166bdaf_0.tar.bz2
+  hash:
+    md5: 30186d27e2c9fa62b45fb1476b7200e3
+    sha256: 150c05a6e538610ca7c43beb3a40d65c90537497a4f6a5f4d15ec0451b6f5ebb
+  category: main
+  optional: false
+- name: krb5
+  version: 1.21.3
+  manager: conda
+  platform: linux-64
+  dependencies:
+    keyutils: '>=1.6.1,<2.0a0'
+    libedit: '>=3.1.20191231,<4.0a0'
+    libgcc-ng: '>=12'
+    libstdcxx-ng: '>=12'
+    openssl: '>=3.3.1,<4.0a0'
+  url: https://conda.anaconda.org/conda-forge/linux-64/krb5-1.21.3-h659f571_0.conda
+  hash:
+    md5: 3f43953b7d3fb3aaa1d0d0723d91e368
+    sha256: 99df692f7a8a5c27cd14b5fb1374ee55e756631b9c3d659ed3ee60830249b238
+  category: main
+  optional: false
+- name: ld_impl_linux-64
+  version: '2.40'
+  manager: conda
+  platform: linux-64
+  dependencies: {}
+  url: https://conda.anaconda.org/conda-forge/linux-64/ld_impl_linux-64-2.40-hf3520f5_7.conda
+  hash:
+    md5: b80f2f396ca2c28b8c14c437a4ed1e74
+    sha256: 764b6950aceaaad0c67ef925417594dd14cd2e22fff864aeef455ac259263d15
+  category: main
+  optional: false
+- name: libcurl
+  version: 8.8.0
+  manager: conda
+  platform: linux-64
+  dependencies:
+    krb5: '>=1.21.3,<1.22.0a0'
+    libgcc-ng: '>=12'
+    libnghttp2: '>=1.58.0,<2.0a0'
+    libssh2: '>=1.11.0,<2.0a0'
+    libzlib: '>=1.2.13,<2.0a0'
+    openssl: '>=3.3.1,<4.0a0'
+    zstd: '>=1.5.6,<1.6.0a0'
+  url: https://conda.anaconda.org/conda-forge/linux-64/libcurl-8.8.0-hca28451_1.conda
+  hash:
+    md5: b8afb3e3cb3423cc445cf611ab95fdb0
+    sha256: 6b5b64cdcdb643368ebe236de07eedee99b025bb95129bbe317c46e5bdc693f3
+  category: main
+  optional: false
+- name: libdeflate
+  version: '1.21'
+  manager: conda
+  platform: linux-64
+  dependencies:
+    __glibc: '>=2.17,<3.0.a0'
+    libgcc-ng: '>=12'
+  url: https://conda.anaconda.org/conda-forge/linux-64/libdeflate-1.21-h4bc722e_0.conda
+  hash:
+    md5: 36ce76665bf67f5aac36be7a0d21b7f3
+    sha256: 728c24ce835700bfdfdf106bf04233fdb040a61ca4ecfd3f41b46fa90cd4f971
+  category: main
+  optional: false
+- name: libedit
+  version: 3.1.20191231
+  manager: conda
+  platform: linux-64
+  dependencies:
+    libgcc-ng: '>=7.5.0'
+    ncurses: '>=6.2,<7.0.0a0'
+  url: https://conda.anaconda.org/conda-forge/linux-64/libedit-3.1.20191231-he28a2e2_2.tar.bz2
+  hash:
+    md5: 4d331e44109e3f0e19b4cb8f9b82f3e1
+    sha256: a57d37c236d8f7c886e01656f4949d9dcca131d2a0728609c6f7fa338b65f1cf
+  category: main
+  optional: false
+- name: libev
+  version: '4.33'
+  manager: conda
+  platform: linux-64
+  dependencies:
+    libgcc-ng: '>=12'
+  url: https://conda.anaconda.org/conda-forge/linux-64/libev-4.33-hd590300_2.conda
+  hash:
+    md5: 172bf1cd1ff8629f2b1179945ed45055
+    sha256: 1cd6048169fa0395af74ed5d8f1716e22c19a81a8a36f934c110ca3ad4dd27b4
+  category: main
+  optional: false
+- name: libffi
+  version: 3.4.2
+  manager: conda
+  platform: linux-64
+  dependencies:
+    libgcc-ng: '>=9.4.0'
+  url: https://conda.anaconda.org/conda-forge/linux-64/libffi-3.4.2-h7f98852_5.tar.bz2
+  hash:
+    md5: d645c6d2ac96843a2bfaccd2d62b3ac3
+    sha256: ab6e9856c21709b7b517e940ae7028ae0737546122f83c2aa5d692860c3b149e
+  category: main
+  optional: false
+- name: libgcc-ng
+  version: 14.1.0
+  manager: conda
+  platform: linux-64
+  dependencies:
+    _libgcc_mutex: '0.1'
+    _openmp_mutex: '>=4.5'
+  url: https://conda.anaconda.org/conda-forge/linux-64/libgcc-ng-14.1.0-h77fa898_0.conda
+  hash:
+    md5: ca0fad6a41ddaef54a153b78eccb5037
+    sha256: b8e869ac96591cda2704bf7e77a301025e405227791a0bddf14a3dac65125538
+  category: main
+  optional: false
+- name: libgomp
+  version: 14.1.0
+  manager: conda
+  platform: linux-64
+  dependencies:
+    _libgcc_mutex: '0.1'
+  url: https://conda.anaconda.org/conda-forge/linux-64/libgomp-14.1.0-h77fa898_0.conda
+  hash:
+    md5: ae061a5ed5f05818acdf9adab72c146d
+    sha256: 7699df61a1f6c644b3576a40f54791561f2845983120477a16116b951c9cdb05
+  category: main
+  optional: false
+- name: libhwloc
+  version: 2.11.1
+  manager: conda
+  platform: linux-64
+  dependencies:
+    __glibc: '>=2.17,<3.0.a0'
+    libgcc-ng: '>=12'
+    libstdcxx-ng: '>=12'
+    libxml2: '>=2.12.7,<3.0a0'
+  url: https://conda.anaconda.org/conda-forge/linux-64/libhwloc-2.11.1-default_hecaa2ac_1000.conda
+  hash:
+    md5: f54aeebefb5c5ff84eca4fb05ca8aa3a
+    sha256: 8473a300e10b79557ce0ac81602506b47146aff3df4cc3568147a7dd07f480a2
+  category: main
+  optional: false
+- name: libiconv
+  version: '1.17'
+  manager: conda
+  platform: linux-64
+  dependencies:
+    libgcc-ng: '>=12'
+  url: https://conda.anaconda.org/conda-forge/linux-64/libiconv-1.17-hd590300_2.conda
+  hash:
+    md5: d66573916ffcf376178462f1b61c941e
+    sha256: 8ac2f6a9f186e76539439e50505d98581472fedb347a20e7d1f36429849f05c9
+  category: main
+  optional: false
+- name: libnghttp2
+  version: 1.58.0
+  manager: conda
+  platform: linux-64
+  dependencies:
+    c-ares: '>=1.23.0,<2.0a0'
+    libev: '>=4.33,<5.0a0'
+    libgcc-ng: '>=12'
+    libstdcxx-ng: '>=12'
+    libzlib: '>=1.2.13,<2.0.0a0'
+    openssl: '>=3.2.0,<4.0a0'
+  url: https://conda.anaconda.org/conda-forge/linux-64/libnghttp2-1.58.0-h47da74e_1.conda
+  hash:
+    md5: 700ac6ea6d53d5510591c4344d5c989a
+    sha256: 1910c5306c6aa5bcbd623c3c930c440e9c77a5a019008e1487810e3c1d3716cb
+  category: main
+  optional: false
+- name: libnsl
+  version: 2.0.1
+  manager: conda
+  platform: linux-64
+  dependencies:
+    libgcc-ng: '>=12'
+  url: https://conda.anaconda.org/conda-forge/linux-64/libnsl-2.0.1-hd590300_0.conda
+  hash:
+    md5: 30fd6e37fe21f86f4bd26d6ee73eeec7
+    sha256: 26d77a3bb4dceeedc2a41bd688564fe71bf2d149fdcf117049970bc02ff1add6
+  category: main
+  optional: false
+- name: libsqlite
+  version: 3.46.0
+  manager: conda
+  platform: linux-64
+  dependencies:
+    libgcc-ng: '>=12'
+    libzlib: '>=1.2.13,<2.0a0'
+  url: https://conda.anaconda.org/conda-forge/linux-64/libsqlite-3.46.0-hde9e2c9_0.conda
+  hash:
+    md5: 18aa975d2094c34aef978060ae7da7d8
+    sha256: daee3f68786231dad457d0dfde3f7f1f9a7f2018adabdbb864226775101341a8
+  category: main
+  optional: false
+- name: libssh2
+  version: 1.11.0
+  manager: conda
+  platform: linux-64
+  dependencies:
+    libgcc-ng: '>=12'
+    libzlib: '>=1.2.13,<2.0.0a0'
+    openssl: '>=3.1.1,<4.0a0'
+  url: https://conda.anaconda.org/conda-forge/linux-64/libssh2-1.11.0-h0841786_0.conda
+  hash:
+    md5: 1f5a58e686b13bcfde88b93f547d23fe
+    sha256: 50e47fd9c4f7bf841a11647ae7486f65220cfc988ec422a4475fe8d5a823824d
+  category: main
+  optional: false
+- name: libstdcxx-ng
+  version: 14.1.0
+  manager: conda
+  platform: linux-64
+  dependencies:
+    libgcc-ng: 14.1.0
+  url: https://conda.anaconda.org/conda-forge/linux-64/libstdcxx-ng-14.1.0-hc0a3c3a_0.conda
+  hash:
+    md5: 1cb187a157136398ddbaae90713e2498
+    sha256: 88c42b388202ffe16adaa337e36cf5022c63cf09b0405cf06fc6aeacccbe6146
+  category: main
+  optional: false
+- name: libuuid
+  version: 2.38.1
+  manager: conda
+  platform: linux-64
+  dependencies:
+    libgcc-ng: '>=12'
+  url: https://conda.anaconda.org/conda-forge/linux-64/libuuid-2.38.1-h0b41bf4_0.conda
+  hash:
+    md5: 40b61aab5c7ba9ff276c41cfffe6b80b
+    sha256: 787eb542f055a2b3de553614b25f09eefb0a0931b0c87dbcce6efdfd92f04f18
+  category: main
+  optional: false
+- name: libxcrypt
+  version: 4.4.36
+  manager: conda
+  platform: linux-64
+  dependencies:
+    libgcc-ng: '>=12'
+  url: https://conda.anaconda.org/conda-forge/linux-64/libxcrypt-4.4.36-hd590300_1.conda
+  hash:
+    md5: 5aa797f8787fe7a17d1b0821485b5adc
+    sha256: 6ae68e0b86423ef188196fff6207ed0c8195dd84273cb5623b85aa08033a410c
+  category: main
+  optional: false
+- name: libxml2
+  version: 2.12.7
+  manager: conda
+  platform: linux-64
+  dependencies:
+    icu: '>=73.2,<74.0a0'
+    libgcc-ng: '>=12'
+    libiconv: '>=1.17,<2.0a0'
+    libzlib: '>=1.2.13,<2.0a0'
+    xz: '>=5.2.6,<6.0a0'
+  url: https://conda.anaconda.org/conda-forge/linux-64/libxml2-2.12.7-hc051c1a_1.conda
+  hash:
+    md5: 340278ded8b0dc3a73f3660bbb0adbc6
+    sha256: 576ea9134176636283ff052897bf7a91ffd8ac35b2c505dfde2890ec52849698
+  category: main
+  optional: false
+- name: libzlib
+  version: 1.2.13
+  manager: conda
+  platform: linux-64
+  dependencies:
+    libgcc-ng: '>=12'
+  url: https://conda.anaconda.org/conda-forge/linux-64/libzlib-1.2.13-h4ab18f5_6.conda
+  hash:
+    md5: 27329162c0dc732bcf67a4e0cd488125
+    sha256: 8ced4afed6322172182af503f21725d072a589a6eb918f8a58135c1e00d35980
+  category: main
+  optional: false
+- name: ncurses
+  version: '6.5'
+  manager: conda
+  platform: linux-64
+  dependencies:
+    libgcc-ng: '>=12'
+  url: https://conda.anaconda.org/conda-forge/linux-64/ncurses-6.5-h59595ed_0.conda
+  hash:
+    md5: fcea371545eda051b6deafb24889fc69
+    sha256: 4fc3b384f4072b68853a0013ea83bdfd3d66b0126e2238e1d6e1560747aa7586
+  category: main
+  optional: false
+- name: openssl
+  version: 3.3.1
+  manager: conda
+  platform: linux-64
+  dependencies:
+    __glibc: '>=2.17,<3.0.a0'
+    ca-certificates: ''
+    libgcc-ng: '>=12'
+  url: https://conda.anaconda.org/conda-forge/linux-64/openssl-3.3.1-h4bc722e_2.conda
+  hash:
+    md5: e1b454497f9f7c1147fdde4b53f1b512
+    sha256: b294b3cc706ad1048cdb514f0db3da9f37ae3fcc0c53a7104083dd0918adb200
+  category: main
+  optional: false
+- name: perl
+  version: 5.32.1
+  manager: conda
+  platform: linux-64
+  dependencies:
+    libgcc-ng: '>=12'
+    libxcrypt: '>=4.4.36'
+  url: https://conda.anaconda.org/conda-forge/linux-64/perl-5.32.1-7_hd590300_perl5.conda
+  hash:
+    md5: f2cfec9406850991f4e3d960cc9e3321
+    sha256: 9ec32b6936b0e37bcb0ed34f22ec3116e75b3c0964f9f50ecea5f58734ed6ce9
+  category: main
+  optional: false
+- name: pigz
+  version: '2.6'
+  manager: conda
+  platform: linux-64
+  dependencies:
+    libgcc-ng: '>=12'
+    libzlib: '>=1.2.13,<2.0.0a0'
+    zlib: ''
+  url: https://conda.anaconda.org/conda-forge/linux-64/pigz-2.6-h2797004_1.conda
+  hash:
+    md5: 5f19233669faf26889f27a2d2710e170
+    sha256: 074bfefe3552545257babaaca0d6e00a5f9732118d0824382c6eb5c7befe7c7e
+  category: main
+  optional: false
+- name: python
+  version: 3.10.14
+  manager: conda
+  platform: linux-64
+  dependencies:
+    bzip2: '>=1.0.8,<2.0a0'
+    ld_impl_linux-64: '>=2.36.1'
+    libffi: '>=3.4,<4.0a0'
+    libgcc-ng: '>=12'
+    libnsl: '>=2.0.1,<2.1.0a0'
+    libsqlite: '>=3.45.2,<4.0a0'
+    libuuid: '>=2.38.1,<3.0a0'
+    libxcrypt: '>=4.4.36'
+    libzlib: '>=1.2.13,<2.0.0a0'
+    ncurses: '>=6.4.20240210,<7.0a0'
+    openssl: '>=3.2.1,<4.0a0'
+    readline: '>=8.2,<9.0a0'
+    tk: '>=8.6.13,<8.7.0a0'
+    tzdata: ''
+    xz: '>=5.2.6,<6.0a0'
+  url: https://conda.anaconda.org/conda-forge/linux-64/python-3.10.14-hd12c33a_0_cpython.conda
+  hash:
+    md5: 2b4ba962994e8bd4be9ff5b64b75aff2
+    sha256: 76a5d12e73542678b70a94570f7b0f7763f9a938f77f0e75d9ea615ef22aa84c
+  category: main
+  optional: false
+- name: python_abi
+  version: '3.10'
+  manager: conda
+  platform: linux-64
+  dependencies: {}
+  url: https://conda.anaconda.org/conda-forge/linux-64/python_abi-3.10-4_cp310.conda
+  hash:
+    md5: 26322ec5d7712c3ded99dd656142b8ce
+    sha256: 456bec815bfc2b364763084d08b412fdc4c17eb9ccc66a36cb775fa7ac3cbaec
+  category: main
+  optional: false
+- name: readline
+  version: '8.2'
+  manager: conda
+  platform: linux-64
+  dependencies:
+    libgcc-ng: '>=12'
+    ncurses: '>=6.3,<7.0a0'
+  url: https://conda.anaconda.org/conda-forge/linux-64/readline-8.2-h8228510_1.conda
+  hash:
+    md5: 47d31b792659ce70f470b5c82fdfb7a4
+    sha256: 5435cf39d039387fbdc977b0a762357ea909a7694d9528ab40f005e9208744d7
+  category: main
+  optional: false
+- name: samtools
+  version: '1.18'
+  manager: conda
+  platform: linux-64
+  dependencies:
+    htslib: '>=1.18,<1.21.0a0'
+    libgcc-ng: '>=12'
+    libzlib: '>=1.2.13,<1.3.0a0'
+    ncurses: '>=6.4,<7.0a0'
+  url: https://conda.anaconda.org/bioconda/linux-64/samtools-1.18-h50ea8bc_1.tar.bz2
+  hash:
+    md5: 7a53b527d66566e2bd8d436766e9c39e
+    sha256: f18a5e13293da2452dcf22c139daeaacebecad7908699764585c03925da1a92d
+  category: main
+  optional: false
+- name: tbb
+  version: 2021.12.0
+  manager: conda
+  platform: linux-64
+  dependencies:
+    __glibc: '>=2.17,<3.0.a0'
+    libgcc-ng: '>=12'
+    libhwloc: '>=2.11.1,<2.11.2.0a0'
+    libstdcxx-ng: '>=12'
+  url: https://conda.anaconda.org/conda-forge/linux-64/tbb-2021.12.0-h434a139_3.conda
+  hash:
+    md5: c667c11d1e488a38220ede8a34441bff
+    sha256: e901e1887205a3f90d6a77e1302ccc5ffe48fd30de16907dfdbdbf1dbef0a177
+  category: main
+  optional: false
+- name: tk
+  version: 8.6.13
+  manager: conda
+  platform: linux-64
+  dependencies:
+    libgcc-ng: '>=12'
+    libzlib: '>=1.2.13,<2.0.0a0'
+  url: https://conda.anaconda.org/conda-forge/linux-64/tk-8.6.13-noxft_h4845f30_101.conda
+  hash:
+    md5: d453b98d9c83e71da0741bb0ff4d76bc
+    sha256: e0569c9caa68bf476bead1bed3d79650bb080b532c64a4af7d8ca286c08dea4e
+  category: main
+  optional: false
+- name: tzdata
+  version: 2024a
+  manager: conda
+  platform: linux-64
+  dependencies: {}
+  url: https://conda.anaconda.org/conda-forge/noarch/tzdata-2024a-h0c530f3_0.conda
+  hash:
+    md5: 161081fc7cec0bfda0d86d7cb595f8d8
+    sha256: 7b2b69c54ec62a243eb6fba2391b5e443421608c3ae5dbff938ad33ca8db5122
+  category: main
+  optional: false
+- name: xz
+  version: 5.2.6
+  manager: conda
+  platform: linux-64
+  dependencies:
+    libgcc-ng: '>=12'
+  url: https://conda.anaconda.org/conda-forge/linux-64/xz-5.2.6-h166bdaf_0.tar.bz2
+  hash:
+    md5: 2161070d867d1b1204ea749c8eec4ef0
+    sha256: 03a6d28ded42af8a347345f82f3eebdd6807a08526d47899a42d62d319609162
+  category: main
+  optional: false
+- name: zlib
+  version: 1.2.13
+  manager: conda
+  platform: linux-64
+  dependencies:
+    libgcc-ng: '>=12'
+    libzlib: 1.2.13
+  url: https://conda.anaconda.org/conda-forge/linux-64/zlib-1.2.13-h4ab18f5_6.conda
+  hash:
+    md5: 559d338a4234c2ad6e676f460a093e67
+    sha256: 534824ea44939f3e59ca8ebb95e3ece6f50f9d2a0e69999fbc692311252ed6ac
+  category: main
+  optional: false
+- name: zstd
+  version: 1.5.6
+  manager: conda
+  platform: linux-64
+  dependencies:
+    libgcc-ng: '>=12'
+    libstdcxx-ng: '>=12'
+    libzlib: '>=1.2.13,<2.0.0a0'
+  url: https://conda.anaconda.org/conda-forge/linux-64/zstd-1.5.6-ha6fb4c9_0.conda
+  hash:
+    md5: 4d056880988120e29d75bfff282e0f45
+    sha256: c558b9cc01d9c1444031bd1ce4b9cff86f9085765f17627a6cd85fc623c8a02b
+  category: main
+  optional: false
diff --git a/modules/nf-core/bowtie2/align/environment.yml b/modules/nf-core/bowtie2/align/environment.yml
index d2796359aeb..68adde8fc36 100644
--- a/modules/nf-core/bowtie2/align/environment.yml
+++ b/modules/nf-core/bowtie2/align/environment.yml
@@ -4,6 +4,9 @@ channels:
   - bioconda
   - defaults
 dependencies:
-  - bioconda::bowtie2=2.5.2
-  - bioconda::samtools=1.18
-  - conda-forge::pigz=2.6
+  # renovate: datasource=conda depName=bioconda/bowtie2
+  - bowtie2=2.5.2
+  # renovate: datasource=conda depName=bioconda/samtools
+  - samtools=1.18
+  # renovate: datasource=conda depName=conda-forge/pigz
+  - pigz=2.6
diff --git a/modules/nf-core/bowtie2/align/main.nf b/modules/nf-core/bowtie2/align/main.nf
index 809525ad36c..9f5480c5175 100644
--- a/modules/nf-core/bowtie2/align/main.nf
+++ b/modules/nf-core/bowtie2/align/main.nf
@@ -2,7 +2,7 @@ process BOWTIE2_ALIGN {
     tag "$meta.id"
     label 'process_high'
 
-    conda "${moduleDir}/environment.yml"
+    conda "${moduleDir}/conda-lock.yml"
     container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
         'https://depot.galaxyproject.org/singularity/mulled-v2-ac74a7f02cebcfcc07d8e8d1d750af9c83b4d45a:f70b31a2db15c023d641c32f433fb02cd04df5a6-0' :
         'biocontainers/mulled-v2-ac74a7f02cebcfcc07d8e8d1d750af9c83b4d45a:f70b31a2db15c023d641c32f433fb02cd04df5a6-0' }"