diff --git a/src/ontology/uberon-edit.obo b/src/ontology/uberon-edit.obo
index ff95ef052e..d91ef26b65 100644
--- a/src/ontology/uberon-edit.obo
+++ b/src/ontology/uberon-edit.obo
@@ -47664,7 +47664,7 @@ consider: FMA:82553
 [Term]
 id: UBERON:0002301
 name: layer of neocortex
-def: "One of the layers of the neocortex." [http://orcid.org/0000-0002-6601-2165]
+def: "One of the layers of the neocortex. These layers are defined by distinctive populations of cells based on somata size, shape, or density, that are aligned into sheets." [http://orcid.org/0000-0002-6601-2165, ISBN-10:0-87893-742-0, PMID:29081739]
 synonym: "cerebral cortex layer" RELATED [MA:0000186]
 synonym: "cortical layer" BROAD []
 synonym: "lamina of neocortex" RELATED [FMA:83138]
@@ -47676,6 +47676,7 @@ xref: FMA:83138
 xref: MA:0000186
 intersection_of: UBERON:0022303 ! nervous system cell part layer
 intersection_of: part_of UBERON:0001950 ! neocortex
+is_a: UBERON:8440004 ! laminar subdivision of the cortex
 
 [Term]
 id: UBERON:0002302
@@ -101770,7 +101771,6 @@ xref: MA:0000902
 xref: NLXANAT:090812
 xref: PBA:294021896
 is_a: UBERON:0002301 ! layer of neocortex
-relationship: develops_from UBERON:0005343 {source="BRAINSPAN"} ! cortical plate
 relationship: immediately_deep_to UBERON:0005394 ! cortical layer V
 
 [Term]
@@ -227788,6 +227788,46 @@ is_a: UBERON:0001744 ! lymphoid tissue
 relationship: part_of UBERON:0010417 ! lymph node T cell domain
 created_by: http://orcid.org/0000-0002-2825-0621
 
+[Term]
+id: UBERON:8440000
+name: cortical layer II/III
+def: "A laminar subdivision of the neocortex that is comprised of both layers II & III." [PMID:34616063]
+synonym: "cerebral cortex layer 2/3" EXACT []
+synonym: "neocortex layer 2/3" EXACT []
+is_a: UBERON:8440004 ! laminar subdivision of the cortex
+
+[Term]
+id: UBERON:8440001
+name: cortical layer IV/V
+def: "A laminar subdivision of the neocortex that is comprised of both layers IV & V." [PMID:34616063]
+synonym: "cerebral cortex layer 4/5" EXACT []
+synonym: "neocortex layer 4/5" EXACT []
+is_a: UBERON:8440004 ! laminar subdivision of the cortex
+
+[Term]
+id: UBERON:8440002
+name: cortical layer V/VI
+def: "A laminar subdivision of the neocortex that is comprised of both layers V & VI." [PMID:34616063]
+synonym: "cerebral cortex layer 5/6" EXACT []
+synonym: "neocortex layer 5/6" EXACT []
+is_a: UBERON:8440004 ! laminar subdivision of the cortex
+
+[Term]
+id: UBERON:8440003
+name: cortical layer VIb
+def: "The deepest layer of the neocortex. While layer 6b is considered part of layer 6, it is distinct morphologically, genetically, and developmentally from the rest of layer 6. Neurons in L6b project to cortical targets and higher-order thalamus and develop from a precursor population that arises from the subplate." [PMID:32160552]
+synonym: "cerebral cortex layer 6b" EXACT []
+synonym: "neocortex layer 6b" EXACT []
+is_a: UBERON:0002301 ! layer of neocortex
+relationship: develops_from UBERON:0004035 ! cortical subplate
+relationship: part_of UBERON:0005395 ! cortical layer VI
+
+[Term]
+id: UBERON:8440004
+name: laminar subdivision of the cortex
+def: "One or a subset of adjacent layers of the neocortex." [ISBN-10:0-87893-742-0]
+is_a: UBERON:0011215 ! central nervous system cell part cluster
+
 [Term]
 id: UBERON:8420000
 name: hair of scalp