Selection of the number of cells used for the NB regression in SCTransform

[BiotechPedro]

Hello everyone 😄

First of all, thank you for the great and easy Seurat framework in general!

By default ncells = 5000 in SCTransform(), but I am asking myself what is the more accurate number of cells for each dataset. For example, I am blindy using the 40% of the cells to build the regression. Do you have some estimates for the best percentage, or it similarly works for a broad range of ncells?

Thank you!