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sc.pl.heatmap var_names function KeyError #3363

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josenimo opened this issue Nov 13, 2024 · 0 comments
Open
3 tasks done

sc.pl.heatmap var_names function KeyError #3363

josenimo opened this issue Nov 13, 2024 · 0 comments
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Bug 🐛 Triage 🩺 This issue needs to be triaged by a maintainer

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@josenimo
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Please make sure these conditions are met

  • I have checked that this issue has not already been reported.
  • I have confirmed this bug exists on the latest version of scanpy.
  • (optional) I have confirmed this bug exists on the main branch of scanpy.

What happened?

I pass a adata.var column header with my gene name that I want displayed, sc.pl.heatmap runs into a key error where my index values are being searched in the genename column

Minimal code sample

sc.pl.heatmap(
    adata=adata,
    var_names=top_25,
    gene_symbols=adata.var.columns.tolist()[2],
    groupby='slide_number',
    swap_axes=True,
    standard_scale='var',
)

Error output

---------------------------------------------------------------------------
KeyError                                  Traceback (most recent call last)
Cell In[86], line 1
----> 1 sc.pl.heatmap(
      2     adata=adata,
      3     var_names=top_25,
      4     gene_symbols=adata.var.columns.tolist()[2],
      5     groupby='slide_number',
      6     swap_axes=True,
      7     standard_scale='var',
      8 )

File /opt/homebrew/Caskroom/mambaforge/base/envs/proteomics/lib/python3.12/site-packages/legacy_api_wrap/__init__.py:80, in legacy_api.<locals>.wrapper.<locals>.fn_compatible(*args_all, **kw)
     77 @wraps(fn)
     78 def fn_compatible(*args_all: P.args, **kw: P.kwargs) -> R:
     79     if len(args_all) <= n_positional:
---> 80         return fn(*args_all, **kw)
     82     args_pos: P.args
     83     args_pos, args_rest = args_all[:n_positional], args_all[n_positional:]

File /opt/homebrew/Caskroom/mambaforge/base/envs/proteomics/lib/python3.12/site-packages/scanpy/plotting/_anndata.py:1120, in heatmap(adata, var_names, groupby, use_raw, log, num_categories, dendrogram, gene_symbols, var_group_positions, var_group_labels, var_group_rotation, layer, standard_scale, swap_axes, show_gene_labels, show, save, figsize, vmin, vmax, vcenter, norm, **kwds)
   1070 """\
   1071 Heatmap of the expression values of genes.
   1072 
   (...)
   1114 tl.rank_genes_groups
   1115 """
   1116 var_names, var_group_labels, var_group_positions = _check_var_names_type(
   1117     var_names, var_group_labels, var_group_positions
   1118 )
-> 1120 categories, obs_tidy = _prepare_dataframe(
   1121     adata,
   1122     var_names,
   1123     groupby,
   1124     use_raw=use_raw,
   1125     log=log,
   1126     num_categories=num_categories,
   1127     gene_symbols=gene_symbols,
   1128     layer=layer,
   1129 )
   1131 # check if var_group_labels are a subset of categories:
   1132 if var_group_labels is not None:

File /opt/homebrew/Caskroom/mambaforge/base/envs/proteomics/lib/python3.12/site-packages/scanpy/plotting/_anndata.py:2057, in _prepare_dataframe(adata, var_names, groupby, use_raw, log, num_categories, layer, gene_symbols)
   2055     groupby.remove(groupby_index)
   2056 keys = list(groupby) + list(np.unique(var_names))
-> 2057 obs_tidy = get.obs_df(
   2058     adata, keys=keys, layer=layer, use_raw=use_raw, gene_symbols=gene_symbols
   2059 )
   2060 assert np.all(np.array(keys) == np.array(obs_tidy.columns))
   2062 if groupby_index is not None:
   2063     # reset index to treat all columns the same way.

File /opt/homebrew/Caskroom/mambaforge/base/envs/proteomics/lib/python3.12/site-packages/scanpy/get/get.py:296, in obs_df(adata, keys, obsm_keys, layer, gene_symbols, use_raw)
    293 else:
    294     alias_index = None
--> 296 obs_cols, var_idx_keys, var_symbols = _check_indices(
    297     adata.obs,
    298     var.index,
    299     dim="obs",
    300     keys=keys,
    301     alias_index=alias_index,
    302     use_raw=use_raw,
    303 )
    305 # Make df
    306 df = pd.DataFrame(index=adata.obs_names)

File /opt/homebrew/Caskroom/mambaforge/base/envs/proteomics/lib/python3.12/site-packages/scanpy/get/get.py:186, in _check_indices(dim_df, alt_index, dim, keys, alias_index, use_raw)
    184         not_found.append(key)
    185 if len(not_found) > 0:
--> 186     raise KeyError(
    187         f"Could not find keys '{not_found}' in columns of `adata.{dim}` or in"
    188         f" {alt_repr}.{alt_search_repr}."
    189     )
    191 return col_keys, index_keys, index_aliases

KeyError: "Could not find keys '[np.str_('O00154'), np.str_('O00515'), np.str_('O94886'), np.str_('P09493'), np.str_('P20338'), np.str_('P23141'), np.str_('P49419'), np.str_('P55268'), np.str_('P98095'), np.str_('P98160'), np.str_('Q01995'), np.str_('Q05682'), np.str_('Q09666'), np.str_('Q13751'), np.str_('Q14195'), np.str_('Q16795'), np.str_('Q8IUX7'), np.str_('Q8WX93'), np.str_('Q96AC1'), np.str_('Q96FZ7'), np.str_('Q96G03'), np.str_('Q9NSE4'), np.str_('Q9NZM1'), np.str_('Q9UN36'), np.str_('Q9Y4K1')]' in columns of `adata.obs` or in adata.var['Genes']."

Versions

# packages in environment at /opt/homebrew/Caskroom/mambaforge/base/envs/proteomics:
#
# Name                    Version                   Build  Channel
adjusttext                1.2.0              pyhd8ed1ab_0    conda-forge
anndata                   0.10.9             pyhd8ed1ab_0    conda-forge
anyio                     4.6.2.post1        pyhd8ed1ab_0    conda-forge
appnope                   0.1.4              pyhd8ed1ab_0    conda-forge
argon2-cffi               23.1.0             pyhd8ed1ab_0    conda-forge
argon2-cffi-bindings      21.2.0          py312h024a12e_5    conda-forge
array-api-compat          1.9                pyhd8ed1ab_0    conda-forge
arrow                     1.3.0              pyhd8ed1ab_0    conda-forge
asttokens                 2.4.1              pyhd8ed1ab_0    conda-forge
async-lru                 2.0.4              pyhd8ed1ab_0    conda-forge
attrs                     24.2.0             pyh71513ae_0    conda-forge
babel                     2.14.0             pyhd8ed1ab_0    conda-forge
beautifulsoup4            4.12.3             pyha770c72_0    conda-forge
bleach                    6.1.0              pyhd8ed1ab_0    conda-forge
brotli                    1.1.0                hd74edd7_2    conda-forge
brotli-bin                1.1.0                hd74edd7_2    conda-forge
brotli-python             1.1.0           py312hde4cb15_2    conda-forge
bzip2                     1.0.8                h99b78c6_7    conda-forge
c-ares                    1.34.2               h7ab814d_0    conda-forge
ca-certificates           2024.8.30            hf0a4a13_0    conda-forge
cached-property           1.5.2                hd8ed1ab_1    conda-forge
cached_property           1.5.2              pyha770c72_1    conda-forge
certifi                   2024.8.30          pyhd8ed1ab_0    conda-forge
cffi                      1.17.1          py312h0fad829_0    conda-forge
charset-normalizer        3.4.0              pyhd8ed1ab_0    conda-forge
colorama                  0.4.6              pyhd8ed1ab_0    conda-forge
comm                      0.2.2              pyhd8ed1ab_0    conda-forge
contourpy                 1.3.0           py312h6142ec9_2    conda-forge
cycler                    0.12.1             pyhd8ed1ab_0    conda-forge
debugpy                   1.8.7           py312hde4cb15_0    conda-forge
decorator                 5.1.1              pyhd8ed1ab_0    conda-forge
defusedxml                0.7.1              pyhd8ed1ab_0    conda-forge
entrypoints               0.4                pyhd8ed1ab_0    conda-forge
exceptiongroup            1.2.2              pyhd8ed1ab_0    conda-forge
executing                 2.1.0              pyhd8ed1ab_0    conda-forge
fonttools                 4.54.1          py312h024a12e_0    conda-forge
fqdn                      1.5.1              pyhd8ed1ab_0    conda-forge
freetype                  2.12.1               hadb7bae_2    conda-forge
get-annotations           0.1.2              pyhd8ed1ab_0    conda-forge
h11                       0.14.0             pyhd8ed1ab_0    conda-forge
h2                        4.1.0              pyhd8ed1ab_0    conda-forge
h5py                      3.11.0          nompi_py312h3cd0824_103    conda-forge
hdf5                      1.14.3          nompi_hec07895_105    conda-forge
hpack                     4.0.0              pyh9f0ad1d_0    conda-forge
httpcore                  1.0.6              pyhd8ed1ab_0    conda-forge
httpx                     0.27.2             pyhd8ed1ab_0    conda-forge
hyperframe                6.0.1              pyhd8ed1ab_0    conda-forge
icu                       75.1                 hfee45f7_0    conda-forge
idna                      3.10               pyhd8ed1ab_0    conda-forge
importlib-metadata        8.5.0              pyha770c72_0    conda-forge
importlib_metadata        8.5.0                hd8ed1ab_0    conda-forge
importlib_resources       6.4.5              pyhd8ed1ab_0    conda-forge
ipykernel                 6.29.5             pyh57ce528_0    conda-forge
ipython                   8.28.0             pyh707e725_0    conda-forge
ipywidgets                8.1.5              pyhd8ed1ab_0    conda-forge
isoduration               20.11.0            pyhd8ed1ab_0    conda-forge
jedi                      0.19.1             pyhd8ed1ab_0    conda-forge
jinja2                    3.1.4              pyhd8ed1ab_0    conda-forge
joblib                    1.4.2              pyhd8ed1ab_0    conda-forge
json5                     0.9.25             pyhd8ed1ab_0    conda-forge
jsonpointer               3.0.0           py312h81bd7bf_1    conda-forge
jsonschema                4.23.0             pyhd8ed1ab_0    conda-forge
jsonschema-specifications 2024.10.1          pyhd8ed1ab_0    conda-forge
jsonschema-with-format-nongpl 4.23.0               hd8ed1ab_0    conda-forge
jupyter                   1.1.1              pyhd8ed1ab_0    conda-forge
jupyter-lsp               2.2.5              pyhd8ed1ab_0    conda-forge
jupyter_client            8.6.3              pyhd8ed1ab_0    conda-forge
jupyter_console           6.6.3              pyhd8ed1ab_0    conda-forge
jupyter_core              5.7.2              pyh31011fe_1    conda-forge
jupyter_events            0.10.0             pyhd8ed1ab_0    conda-forge
jupyter_server            2.14.2             pyhd8ed1ab_0    conda-forge
jupyter_server_terminals  0.5.3              pyhd8ed1ab_0    conda-forge
jupyterlab                4.2.5              pyhd8ed1ab_0    conda-forge
jupyterlab_pygments       0.3.0              pyhd8ed1ab_1    conda-forge
jupyterlab_server         2.27.3             pyhd8ed1ab_0    conda-forge
jupyterlab_widgets        3.0.13             pyhd8ed1ab_0    conda-forge
kaleido                   0.2.1                    pypi_0    pypi
kiwisolver                1.4.7           py312h6142ec9_0    conda-forge
krb5                      1.21.3               h237132a_0    conda-forge
lcms2                     2.16                 ha0e7c42_0    conda-forge
legacy-api-wrap           1.4                pyhd8ed1ab_1    conda-forge
lerc                      4.0.0                h9a09cb3_0    conda-forge
libaec                    1.1.3                hebf3989_0    conda-forge
libblas                   3.9.0           24_osxarm64_openblas    conda-forge
libbrotlicommon           1.1.0                hd74edd7_2    conda-forge
libbrotlidec              1.1.0                hd74edd7_2    conda-forge
libbrotlienc              1.1.0                hd74edd7_2    conda-forge
libcblas                  3.9.0           24_osxarm64_openblas    conda-forge
libcurl                   8.10.1               h13a7ad3_0    conda-forge
libcxx                    19.1.2               ha82da77_0    conda-forge
libdeflate                1.22                 hd74edd7_0    conda-forge
libedit                   3.1.20191231         hc8eb9b7_2    conda-forge
libev                     4.33                 h93a5062_2    conda-forge
libexpat                  2.6.3                hf9b8971_0    conda-forge
libffi                    3.4.2                h3422bc3_5    conda-forge
libgfortran               5.0.0           13_2_0_hd922786_3    conda-forge
libgfortran5              13.2.0               hf226fd6_3    conda-forge
libhwloc                  2.11.1          default_h7685b71_1000    conda-forge
libiconv                  1.17                 h0d3ecfb_2    conda-forge
libjpeg-turbo             3.0.0                hb547adb_1    conda-forge
liblapack                 3.9.0           24_osxarm64_openblas    conda-forge
libllvm14                 14.0.6               hd1a9a77_4    conda-forge
libnghttp2                1.58.0               ha4dd798_1    conda-forge
libopenblas               0.3.27          openmp_h517c56d_1    conda-forge
libpng                    1.6.44               hc14010f_0    conda-forge
libsodium                 1.0.20               h99b78c6_0    conda-forge
libsqlite                 3.46.1               hc14010f_0    conda-forge
libssh2                   1.11.0               h7a5bd25_0    conda-forge
libtiff                   4.7.0                hfce79cd_1    conda-forge
libwebp-base              1.4.0                h93a5062_0    conda-forge
libxcb                    1.17.0               hdb1d25a_0    conda-forge
libxml2                   2.12.7               h01dff8b_4    conda-forge
libzlib                   1.3.1                h8359307_2    conda-forge
littleutils               0.2.4              pyhd8ed1ab_0    conda-forge
llvm-openmp               19.1.1               hb52a8e5_1    conda-forge
llvmlite                  0.43.0          py312ha9ca408_1    conda-forge
loguru                    0.7.2           py312h81bd7bf_2    conda-forge
markupsafe                3.0.1           py312h906988d_1    conda-forge
matplotlib                3.9.2           py312h1f38498_1    conda-forge
matplotlib-base           3.9.2           py312h9bd0bc6_1    conda-forge
matplotlib-inline         0.1.7              pyhd8ed1ab_0    conda-forge
mistune                   3.0.2              pyhd8ed1ab_0    conda-forge
mpmath                    1.3.0              pyhd8ed1ab_0    conda-forge
munkres                   1.1.4              pyh9f0ad1d_0    conda-forge
natsort                   8.4.0              pyhd8ed1ab_0    conda-forge
nbclient                  0.10.0             pyhd8ed1ab_0    conda-forge
nbconvert-core            7.16.4             pyhd8ed1ab_1    conda-forge
nbformat                  5.10.4             pyhd8ed1ab_0    conda-forge
ncurses                   6.5                  h7bae524_1    conda-forge
nest-asyncio              1.6.0              pyhd8ed1ab_0    conda-forge
networkx                  3.4.1              pyhd8ed1ab_0    conda-forge
notebook                  7.2.2              pyhd8ed1ab_0    conda-forge
notebook-shim             0.2.4              pyhd8ed1ab_0    conda-forge
numba                     0.60.0          py312h41cea2d_0    conda-forge
numpy                     2.0.2           py312h801f5e3_0    conda-forge
openjpeg                  2.5.2                h9f1df11_0    conda-forge
openssl                   3.3.2                h8359307_0    conda-forge
outdated                  0.2.2              pyhd8ed1ab_1    conda-forge
overrides                 7.7.0              pyhd8ed1ab_0    conda-forge
packaging                 24.1               pyhd8ed1ab_0    conda-forge
pandas                    2.2.3           py312hcd31e36_1    conda-forge
pandas-flavor             0.6.0              pyhd8ed1ab_2    conda-forge
pandocfilters             1.5.0              pyhd8ed1ab_0    conda-forge
parso                     0.8.4              pyhd8ed1ab_0    conda-forge
patsy                     0.5.6              pyhd8ed1ab_0    conda-forge
pexpect                   4.9.0              pyhd8ed1ab_0    conda-forge
pickleshare               0.7.5                   py_1003    conda-forge
pillow                    11.0.0          py312haf37ca6_0    conda-forge
pingouin                  0.5.5              pyhd8ed1ab_0    conda-forge
pip                       24.2               pyh8b19718_1    conda-forge
pkgutil-resolve-name      1.3.10             pyhd8ed1ab_1    conda-forge
platformdirs              4.3.6              pyhd8ed1ab_0    conda-forge
plotly                    5.24.1             pyhd8ed1ab_0    conda-forge
prometheus_client         0.21.0             pyhd8ed1ab_0    conda-forge
prompt-toolkit            3.0.48             pyha770c72_0    conda-forge
prompt_toolkit            3.0.48               hd8ed1ab_0    conda-forge
psutil                    6.0.0           py312h0bf5046_2    conda-forge
pthread-stubs             0.4               hd74edd7_1002    conda-forge
ptyprocess                0.7.0              pyhd3deb0d_0    conda-forge
pure_eval                 0.2.3              pyhd8ed1ab_0    conda-forge
pycparser                 2.22               pyhd8ed1ab_0    conda-forge
pygments                  2.18.0             pyhd8ed1ab_0    conda-forge
pynndescent               0.5.13             pyhff2d567_0    conda-forge
pyobjc-core               10.3.1          py312hd24fc31_1    conda-forge
pyobjc-framework-cocoa    10.3.1          py312hd24fc31_1    conda-forge
pyparsing                 3.2.0              pyhd8ed1ab_1    conda-forge
pysocks                   1.7.1              pyha2e5f31_6    conda-forge
python                    3.12.7          h739c21a_0_cpython    conda-forge
python-dateutil           2.9.0              pyhd8ed1ab_0    conda-forge
python-fastjsonschema     2.20.0             pyhd8ed1ab_0    conda-forge
python-json-logger        2.0.7              pyhd8ed1ab_0    conda-forge
python-tzdata             2024.2             pyhd8ed1ab_0    conda-forge
python_abi                3.12                    5_cp312    conda-forge
pytz                      2024.1             pyhd8ed1ab_0    conda-forge
pyyaml                    6.0.2           py312h024a12e_1    conda-forge
pyzmq                     26.2.0          py312hf8a1cbd_3    conda-forge
qhull                     2020.2               h420ef59_5    conda-forge
readline                  8.2                  h92ec313_1    conda-forge
referencing               0.35.1             pyhd8ed1ab_0    conda-forge
requests                  2.32.3             pyhd8ed1ab_0    conda-forge
rfc3339-validator         0.1.4              pyhd8ed1ab_0    conda-forge
rfc3986-validator         0.1.1              pyh9f0ad1d_0    conda-forge
rpds-py                   0.20.0          py312he431725_1    conda-forge
scanpy                    1.10.3             pyhd8ed1ab_0    conda-forge
scikit-learn              1.5.2           py312h387f99c_1    conda-forge
scipy                     1.14.1          py312heb3a901_0    conda-forge
seaborn                   0.13.2               hd8ed1ab_2    conda-forge
seaborn-base              0.13.2             pyhd8ed1ab_2    conda-forge
send2trash                1.8.3              pyh31c8845_0    conda-forge
session-info              1.0.0              pyhd8ed1ab_0    conda-forge
setuptools                75.1.0             pyhd8ed1ab_0    conda-forge
six                       1.16.0             pyh6c4a22f_0    conda-forge
sniffio                   1.3.1              pyhd8ed1ab_0    conda-forge
soupsieve                 2.5                pyhd8ed1ab_1    conda-forge
stack_data                0.6.2              pyhd8ed1ab_0    conda-forge
statsmodels               0.14.4          py312h755e627_0    conda-forge
stdlib-list               0.11.0             pyhd8ed1ab_0    conda-forge
tabulate                  0.9.0              pyhd8ed1ab_1    conda-forge
tbb                       2021.13.0            h7b3277c_0    conda-forge
tenacity                  9.0.0              pyhd8ed1ab_0    conda-forge
terminado                 0.18.1             pyh31c8845_0    conda-forge
threadpoolctl             3.5.0              pyhc1e730c_0    conda-forge
tinycss2                  1.3.0              pyhd8ed1ab_0    conda-forge
tk                        8.6.13               h5083fa2_1    conda-forge
tomli                     2.0.2              pyhd8ed1ab_0    conda-forge
tornado                   6.4.1           py312h024a12e_1    conda-forge
tqdm                      4.66.5             pyhd8ed1ab_0    conda-forge
traitlets                 5.14.3             pyhd8ed1ab_0    conda-forge
types-python-dateutil     2.9.0.20241003     pyhff2d567_0    conda-forge
typing-extensions         4.12.2               hd8ed1ab_0    conda-forge
typing_extensions         4.12.2             pyha770c72_0    conda-forge
typing_utils              0.1.0              pyhd8ed1ab_0    conda-forge
tzdata                    2024b                hc8b5060_0    conda-forge
umap-learn                0.5.6           py312h81bd7bf_1    conda-forge
uri-template              1.3.0              pyhd8ed1ab_0    conda-forge
urllib3                   2.2.3              pyhd8ed1ab_0    conda-forge
wcwidth                   0.2.13             pyhd8ed1ab_0    conda-forge
webcolors                 24.8.0             pyhd8ed1ab_0    conda-forge
webencodings              0.5.1              pyhd8ed1ab_2    conda-forge
websocket-client          1.8.0              pyhd8ed1ab_0    conda-forge
wheel                     0.44.0             pyhd8ed1ab_0    conda-forge
widgetsnbextension        4.0.13             pyhd8ed1ab_0    conda-forge
xarray                    2024.9.0           pyhd8ed1ab_1    conda-forge
xorg-libxau               1.0.11               hd74edd7_1    conda-forge
xorg-libxdmcp             1.1.5                hd74edd7_0    conda-forge
xz                        5.2.6                h57fd34a_0    conda-forge
yaml                      0.2.5                h3422bc3_2    conda-forge
zeromq                    4.3.5                h9f5b81c_6    conda-forge
zipp                      3.20.2             pyhd8ed1ab_0    conda-forge
zstandard                 0.23.0          py312h15fbf35_1    conda-forge
zstd                      1.5.6                hb46c0d2_0    conda-forge
@josenimo josenimo added Bug 🐛 Triage 🩺 This issue needs to be triaged by a maintainer labels Nov 13, 2024
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