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Mmseq does not cluster: Segmentation fault (core dumped) #928

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loopersosa opened this issue Jan 15, 2025 · 0 comments
Open

Mmseq does not cluster: Segmentation fault (core dumped) #928

loopersosa opened this issue Jan 15, 2025 · 0 comments

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@loopersosa
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Expected Behavior

I am new to this.

The mmseqs easy-cluster command should successfully cluster the sequences in the provided FASTA file (cdr_sequences.fasta) without crashing.

Current Behavior

The mmseqs easy-cluster command fails with a segmentation fault (core dumped) and the following errors:

Segmentation fault (core dumped)
Error: Prefilter step 0 died
Error: Search died

##MMseqs Output (for bugs)
The complete output of the mmseqs command is:

Time for index table init: 0h 0m 0s 385ms
Process prefiltering step 1 of 1

k-mer similarity threshold: 154
Starting prefiltering scores calculation (step 1 of 1)
Query db start 1 to 2979
Target db start 1 to 2979
Segmentation fault (core dumped)                                  ] 0.00% 1 eta -
Error: Prefilter step 0 died
Error: Search died

Context

I am trying to cluster sequences from a FASTA file (cdr_sequences.fasta) using mmseqs as part of a larger script for processing antibody sequences. The segmentation fault occurs during the prefiltering step.

this is the debugging window:
image

MMseqs2 Version: 16.747c6

this is the memory:

total used free shared buff/cache available
Mem: 62Gi 6.1Gi 19Gi 1.3Gi 38Gi 56Gi
Swap: 8.0Gi 2.0Gi 6.0Gi

Description: Ubuntu 24.04.1 LTS

i thought it is good to include this one as well:
metadata of all_summary.tsv

4nc1	D	NA	0	B	protein		cell wall-binding repeat protein	IMMUNE SYSTEM	10/23/13	Crystal Structure of TcdA-A2 Bound to A20.1 VHH and A26.8 VHH	CLOSTRIDIUM DIFFICILE; LAMA GLAMA	lama glama		clostridium difficile	Murase, T., Eugenio, L., Schorr, M., Hussack, G., Tanha, J., Kitova, E.N., Klassen, J.S., Ng, K.K.S.	2.61	X-RAY DIFFRACTION	0.252	0.202	False	True	IGHV1			None	None	None	None	None
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