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Determine how to handle files with all reads <100bp #5

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adswafford opened this issue Aug 25, 2021 · 0 comments
Open

Determine how to handle files with all reads <100bp #5

adswafford opened this issue Aug 25, 2021 · 0 comments

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@adswafford
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In Qiita study https://qiita.ucsd.edu/study/description/13962 the default workflow failed with the error message:
1 validator jobs failed: Validator c4ce42b1-4cde-4cc4-b4de-f8010c5c8da1 error message: Some of the files are empty: SAMEA2582027.ERR527149.R1.ebi.fastq.gz, SAMEA2582059.ERR527181.R1.ebi.fastq.gz, SAMEA2582062.ERR527184.R1.ebi.fastq.gz, SAMEA2582076.ERR527198.R1.ebi.fastq.gz, SAMEA2582154.ERR528296.R1.ebi.fastq.gz, SAMEA2582027.ERR527149.R2.ebi.fastq.gz, SAMEA2582059.ERR527181.R2.ebi.fastq.gz, SAMEA2582062.ERR527184.R2.ebi.fastq.gz, SAMEA2582076.ERR527198.R2.ebi.fastq.gz, SAMEA2582154.ERR528296.R2.ebi.fastq.gz

These files, located at /panfs/panfs1.ucsd.edu/panscratch/qiita/qebil/PRJEB6337/ do not appear to be empty but rather have no sequences >=100 bp resulting in the appearance of empty files from fastp. Need to consider whether to capture and handle files that will not pass filter, likely by writing to a separate prep.

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