Annotation Features

You can either import GFF3 or the Jalview feature format

The parse is implemented as biojs-io-gff.

Small gotchas

• indexes start at 0

How to add features/annotations to the MSA

var xhr = require("xhr");
var gff = msa.io.gff;
xhr("./data/fer1.gff3", function(err, request, body) {
  var features = gffParser.parseSeqs(body);
  msaInstance.seqs.addFeatures(features);
});

• you can choose your favorite library to do the XHR-Request
• you can add the features independently to the loading of the sequences
• you can remove all features by msa.seqs.removeAllFeatures()
• instead of msa.io.gff is the convenient form for require("biojs-io-gff") `

Jalview feature format

domain	red
coil	cccccc
Your Own description here	FER_CAPAA	-1	3	93	domain
Your Own description here	FER_CAPAN	-1	48	144	chain
startgroup	secondarystucture
PDB secondary structure annotation	FER1_SPIOL	-1	52	59	strand
PDB secondary structure annotation	FER1_SPIOL	-1	74	80	helix
endgroup	secondarystructure

<description> <id> <sequenceIndex(opt.)> <start> <end> <type> <scopt(opt.)>

• tab-separated file
• this format doesn't allow comments
• a sequence index of -1: access by ID

more

GFF3

##gff-version 3
ctg123 . gene            10  90  .  +  .  Name=EDEN;Color=green
ctg123 . TF_binding_site 20  32  .  +  .  Name=TF binding site;Color=red
ctg123 . mRNA            60  65  .  +  .  Name=EDEN.2;Color=blue
ctg123 . mRNA            13  40  .  +  .  Parent=gene00001;Name=EDEN.3;ID=mRNA00003

<id> <source(opt)> <type> <start> <stop> <score(opt)> <strand(opt)> <phase(opt)> <attributes(opt)>

• tab-separated file
• . is undefined
• the header ##gff-version 3 is required
• comments will be ignored
• define

• colors with Color
• text with Name

• Parent and ID are optional

more