NEWS.md

Signac 1.9.0

Bug fixes:

• Fixed Motif object subsetting when using only one feature (#1266)
• Avoid error in chromVAR when matrix contains NA values (#1254)
• Fixed error in CoveragePlot() when setting show.bulk=TRUE (#1294)

Other changes:

• Improved error message when no fragment file information stored (#1236)
• Add tol parameter to RunSVD() to control the irlba::irlba() tol parameter
• Added split.by parameters to CoveragePlot() and PlotFootprint() (#523)

Signac 1.8.0

Bug fixes:

• Fixed bug in Motif object subsetting (#1162)
• Fixed bug in FeatureMatrix() where cell names would not be converted correctly when running with cells=NULL (#1198)
• Fixed bug causing crash when fragment file line lengths are very large (#723)

Other changes:

• Improved fold change calculation (#1174)
• Added subset() method for Fragment-class objects
• Set random seed within CoverageTrack to enable reproducible coverage plots (#1206)
• Add ability to plot region using gene name in PeakPlot(), LinkPlot(), and AnnotationPlot() (#919)
• Change Motif class definition to allow any CsparseMatrix in the data slot

Signac 1.7.0

New functionality:

• Added method parameter to LinkPeaks() (@saketkc; #1030)
• Added ability to plot transcript isoforms (set annotation="transcript" in CoveragePlot())
• Added multiple testing correction to FindMotifs(), and new p.adjust column in output dataframe
• Added p.adjust.method parameter to FindMotifs() to control multiple testing correction method used

Bug fixes:

• Fixed bug in CallPeaks() when project name contained whitespace (#981)
• Fixed bug in CoveragePlot() when tile=TRUE
• Fixed bug in InsertionBias() that set the coordinates beyond the end of some chromosomes (#986)
• Fixed bug in BigwigTrack() when supplying a single bigwig file (#1053)
• Fixed bug in GeneActivity() when specifying biotypes (#1058)
• Fixed bug in GeneActivity() when gene name is an empty string (#1055)
• Fixed bug in FeatureMatrix() when using list of Fragment objects (#1056)
• Fixed bug in RegionMatrix() when running on objects containing renamed cells (#1076)
• Fixed bug in Footprint() when using a FASTA file (#1092)
• Fixed bug in Footprint() when using list of genomic regions (#1098)
• Add check for invalid features in FindMotifs() (#1109)

Other changes:

• Improved error message when no fragment file is supplied in CallPeaks() (#1062)
• Moved Seurat, ggforce, ggrepel, ggseqlogo, lsa, qlcMatrix to suggested packages
• Added progress bar to GetGRangesFromEnsDb()

Signac 1.6.0

New functionality:

• Added RegionHeatmap() function
• Added RegionPlot() function
• Added RegionMatrix() function
• Added MotifCounts() function
• Added ability to plot multiple assays simultaneously in CoveragePlot() by providing a list of assay names
• Added assay.scale, bigwig.scale, and split.assay parameters to CoveragePlot()
• Added new option to provide quantile ymax value in CoveragePlot()
• Added bigwig.scale and ymax parameters to BigwigTrack()
• Added peak.slot parameter to LinkPeaks() (#932)

Bug fixes:

• Fixed behaviour of min.features parameter in CreateChromatinAssay() to retain cells with >= min.features (#902)
• Fixed behaviour of min.cells parameter in LinkPeaks() (#932)

Other changes:

• Updated documentation for CoveragePlot() (#992)
• Removed method parameter from LinkPeaks() (#932)

Signac 1.5.0

Bug fixes:

• Fixed bug in FeatureMatrix() when cells information not present in Fragment object (#803)
• Fixed bug in object merging (#804)
• Add ability to run LinkPeaks() using Ensembl IDs (#858)
• Fix issue in GeneActivity() when gene names are NA (#865)
• Fix bug in FeatureMatrix() when only one region supplied
• Allow negative values in ExpressionPlot() when using scaled data (#893)

Other changes:

• Added idf parameter to RunTFIDF() to use precomputed IDF vector
• Added gene.id parameter to GeneActivity() to allow output genes named using gene ID (#837)
• Added sep parameter to ConnectionsToLinks() (#841)

Signac 1.4.0

New functionality:

• Added ability to display multiple assays in CoveragePlot(). The assay parameter can now be a list of assays to plot data from, with signal colored by assay of origin.

Bug fixes:

• Fixed bug in FindMotifs() when using only one region as input (#732)
• Add check for correct number of columns in fragment file (#748)
• Fixed gene lookup when annotations contain NA values (#771)
• Fixed error in ClosestFeature() when query contained regions on contigs not present in gene annotation (#758)
• Fixed bug in TSSEnrichment() when using multiple fragment files (#783)
• Fixed bug in CallPeaks() when multiple fragment files used as input
• Fixed bug in CallPeaks() to account for 0-based starts in called peaks
• Fixed bug in gene name lookup when gene names contain - characters (#759)

Other changes:

• Updated documentation for genome parameter in AddMotifs() and RunChromVAR() (#712)
• Updated the FoldChange() function to use normalized counts rather than raw counts (#795)
• Improved error checking in GeneActivity() (#797)
• Added format parameter to CallPeaks() (#682)

Signac 1.3.0

Bug fixes:

• Fixed LinkPeaks() function when running on a single gene (#629)
• Added fragment.tempdir parameter to CallPeaks() to enable setting directory that split fragment files are written to during peak calling (#579)
• Fixed error in FeatureMatrix() when setting sep parameter (#626)
• Fixed peak calling error when group names contain special characters
• Fixed issue with RenameCells() when cell information not present in Fragment object (#704)

Other changes:

• Improved error checking for GeneActivity() (#625)
• Added FoldChange() method for ChromatinAssay() object that sets proper parameters for chromatin data. This fixes the calculation of fold changes when running Seurat::FindMarkers() on single-cell chromatin data.

Signac 1.2.1

New functionality:

• Added head() method for Fragment-class objects.

Bug fixes:

• Fixed bug in ChromatinAssay merging (#596)

Other changes:

• Added support for fragment files containing headers (cellranger-atac v2; #609)

Signac 1.2.0

New functionality:

• Added BigwigTrack() function to plot data from bigWig files
• Added bigwig and bigwig.type arguments to CoveragePlot() to include bigWig files in CoveragePlot()
• Added region.highlight parameter to CoveragePlot()
• Added biotypes parameter to GeneActivity() and GetTSSPositions() functions
• Added max.width parameter to GeneActivity()
• Added min.distance parameter to LinkPeaks() (#561)

Bug fixes:

• Fixed fragment file reading when only one fragment found in requested region (#474)
• Fixed standard.chromosomes parameter in GetGRangesFromEnsDb() (#513)
• Fixed group.by parameter in PlotFootprint() (#522)
• Fixed bug that would cause some gene coordinates used by GeneActivity() to be incorrect (#521)
• Fixed error message in FindMotifs() (#549)
• Fixed bug in CountsInRegion() (#563)

Other changes:

• Improved speed of ChromatinAssay merging
• Improved error message for TSSEnrichment() (#485)
• Improved error messages when trying to run ChromatinAssay-specific functions on non-ChromatinAssay assays
• Performance improvements
• Changed default value for n in NucleosomeSignal()
• Enabled parallization in TSSEnrichment() when fast=TRUE
• Added early error checking in LinkPeaks() (#550)
• Change to sparse matrix correlation in LinkPeaks() (#550)
• Moved biovizBase and Biostrings to suggested packages
• Removed ggbio dependency
• Re-implemented AnnotationPlot()

Signac 1.1.1

New functionality:

• Added group.by parameter to PeakPlot() to allow coloring plotted genomic ranges by metadata variables.
• Added peaks.group.by and ranges.group.by parameters to CoveragePlot() to allow coloring plotted genomic ranges in CoveragePlot() to be colored by metadata variables.

Bug fixes:

• Update meta feature information (overall peak accessibility) when subsetting objects to avoid counts becoming inaccurate (#332)
• Prevent dropping features when creating a merged ChromatinAssay (#340)
• Fix compilation error when using g++ version <5 (#326)
• Retain motif positions during subset (#364)
• Fix assay parameter in CoveragePlot()
• Fix error when merging ChromatinAssay object (#355)
• Add more informative error message when all features or cells removed by parameter choices in CreateChromatinAssay() (#387)
• Fix bug in CreateChromatinAssay() when setting both min.cells and min.features arguments (#390)
• Improved support for remote fragment files
• Fixed bug in PlotFootprint() when only one cell in an identity class (#406)

Other changes:

• Added citation information to the package
• Added SeuratObject dependency

Signac 1.1.0

New functionality:

• Added CallPeaks() function to call peaks using MACS2. Peaks can be called for different groups of cells separately by setting the group.by parameter
• Added LinkPeaks() function to link peaks to correlated genes.
• Added AddMotifs() function to add motif information to a Seurat object or ChromatinAssay.
• Added AggregateTiles() function to combine adjacent genome tiles
• Added ranges parameter to CoveragePlot() to plot addition sets of genomic ranges
• Added show.bulk parameter to CoveragePlot() to plot accessibility of all cells combined
• Added ability to remove Fragment objects and modify the file path for existing fragment objects (#206)

Bug fixes:

• Fixed bugs in AlleleFreq() (#196 and #260)
• Fixed bug in FeatureMatrix() (#205, #291)
• Fixed bug in CreateChromatinAssay() when setting min.features argument (#194)
• Fixed bug in CreateChromatinAssay() when setting min.cells argument (#292)
• Fixed bug in TSSEnrichment() when cell information not set for fragment files (#203)
• Fixed bug in TSSEnrichment() when no fragments present in TSS region (#244)
• Removed qvalue calculation from FindMotifs() (#223)
• Fixed bug in SetAssayData() when setting the scale.data slot

Other changes:

• Improved feature matching in MatchRegionStats() function when matching distribution of multiple features (eg, GC content and overall accessibility)
• Changed parameter names in MatchRegionStats()

Signac 1.0.0

This release includes major updates to the Signac package, including new functionality, performance improvements, and new data structures.

The entire package has been updated to use the new ChromatinAssay class for the storage of single-cell chromatin data. This is an extension of the standard Seurat Assay that adds additional slots needed for the analysis of chromatin data, including genomic ranges, genome information, fragment file information, motifs, gene annotations, and genomic links.

In addition, we have defined a new Fragment class to store information relating to a fragment file. This makes use of the fragment files within Signac more robust, as checks are now performed to verify that the expected cells are present in the fragment file, and that the fragment file or index are not modified on disk.

Key new functionality:

• Store multiple fragment files: you can now store as many fragment files as needed in a single object, and all functions that use the fragment file will pull data from each of the files. Cell barcodes in the fragment files do not need to match the cell barcodes in the object.
• Use remote fragment files: you can now use all the same functionality with fragment files hosted on remote servers accessible through http or ftp.
• Transcription factor footprinting: New Footprint() and PlotFootprint() functions for TF footprinting analysis.
• Bioconductor methods: call granges(), findOverlaps(), seqinfo(), and other Bioconductor generic functions directly on the ChromatinAssay or Seurat object.
• New multi-modal visualization methods: Jointly visualize RNA expression and chromatin accessibility using the CoveragePlot() function.
• New interactive visualizations: Interactively browse the genome using the CoverageBrowser() function.
• Mitochondrial lineage tracing: New functions to identify informative mitochondrial alleles, find clonotypes, and predict cell lineage relationships using mitochondrial mutations.

Other changes:

• Updates to NucleosomeSignal(): we have greatly improved the scalability of NucleosomeSignal(), and fixed a bug present in previous versions. The score computed by NucleosomeSignal() in 1.0.0 will be different to that computed by previous versions of Signac.
• New CountFragments() function: a fast, memory-efficient function implemented in C++ that counts the total number of fragments for each cell barcode present in a fragment file.
• New fast option in the TSSEnrichment() function. Setting this to TRUE will compute the TSS enrichment score per cell without storing the entire cell by TSS position matrix. This can significantly reduce memory requirements for large datasets, but does not allow subsequent plotting of the TSS signal for different groups of cells.
• New TilePlot() function and tile parameter for CoveragePlot() to plot Tn5 integration events in a genomic region for individual cells.
• Performance improvements for FeatureMatrix(), CoveragePlot(), and TSSEnrichment()
• Added the manually curated hg38 genomic blacklist regions curated by Anshul Kundaje and Anna Shcherbina. These are available as the blacklist_hg38_unified object.
• Updated the FRiP() function to use total fragment counts per cell stored in object metadata.

Signac 0.2.5

• New DepthCor function to compute the correlation between sequencing depth and reduced dimension components.
• Performance improvements for RunTFIDF.
• Removed option to use EnsDb object in ClosestFeatures and CoveragePlot. Use GRanges instead.
• Removed ucsc parameter from CoveragePlot.
• Fixed bug in FeatureMatrix that would cause fragments to be counted multiple times if nchunk was greater than the number of features used.
• Fixed bug in CoveragePlot that would prevent plotting multiple regions when using GRanges.
• Fixed bug in CoveragePlot that would prevent plotting when a different assay was active.
• Removed dependencies: GenomicFeatures
• Moved dependencies to suggests: Biostrings, BSgenome
• Removed from suggests: BSgenome.Hsapiens.UCSC.hg19, EnsDb.Hsapiens.v75, JASPAR2018

Signac 0.2.4

• First CRAN release.
• New SubsetMatrix function to subset a matrix based on number of non-zero elements in the rows or columns.
• Removed seed.use parameter from RunSVD.

Signac 0.2.3

• New UnifyPeaks function to create a merged set of peaks from multiple samples.

Signac 0.2.2

• Bug fix for RunSVD: previously, scaling was applied to each cell rather than each component. Now, mean centering and SD scaling are applied to the cell embeddings within a component.
• Added scale.embeddings option to RunSVD to control whether embeddings are scaled and centered.
• Added irlba.work parameter to RunSVD.
• Update to allow comment characters in fragment file cell names

Signac 0.2.1

• Removed SingleCoveragePlot from exported functions
• Added executable examples for all functions
• Store raw SVD output in DimReduc misc slot in RunSVD
• Fixed strand orientation for gene plot in CoveragePlot
• Fix missing x-axis when plotting peaks but not genes in CoveragePlot

Signac 0.2.0

• Removed dependency on TFBSTools, motifmatchr, AnnotationDbi, ggbio, AnnotationFilter
• Renamed PeriodPlot to FragmentHistogram
• Removed motif dimension reduction functions
• Removed motif clustering functions
• Removed neighbors and reductions slots from motif class
• Added motif.names slot to motif class
• Added ability to plot peak ranges in CoveragePlot
• Added ability to plot gene annotations from GRanges object
• Changed gene plot style in CoveragePlot
• Allow passing additional arguments to FilterFragments
• Add inst/extdata
• Change DESCRIPTION file so that Bioconductor dependencies are automatically installed

Signac 0.1.6

• Bug fix for GetCellsInRegion
• Improve documentation

Signac 0.1.5

• New TSSEnrichment and TSSPlot functions for TSS enrichment scoring
• New InsertionBias function
• New options in CoveragePlot for scaling tracks
• Major speed improvements for CoveragePlot
• Improved documentation (added examples)

Signac 0.1.4

• Updates to CoveragePlot: now plots a Tn5 integration score per base, rather than the whole fragment.

Signac 0.1.3

• New GetIntersectingFeatures function to find overlapping peaks between objects
• New MergeWithRegions function to perform region-aware Seurat object merging

Signac 0.1.2

• New RunChromVAR function to run chromVAR through Signac
• New RegionStats function to add statistics about peak sequences to the feature metadata
• Improvements to FindMotifs: now selects a set of background peaks matching the sequence characteristics of the input

Signac 0.1.1

• Added IntersectMatrix
• Added unit tests
• Bug fixes for ChunkGRanges

Signac 0.1.0

• This is the first release of Signac!