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21st - 22nd October 2024, Francis Crick Institute, London

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Overview

In this workshop, we will bridge the gap between advanced microscopy data generation and the computational skills required for its analysis. By leveraging open-source tools like FIJI and napari, participants will learn to automate image analysis, enhancing the precision, efficiency, and reproducibility of their research. This two-day event, led by experienced core facility staff from the Francis Crick Institute and King's College London, offers a practical approach to mastering quantitative analysis and workflow automation, essential for advancing research across multiple domains.

Applications for this workshop have now closed.

Instructors

Preparation

  1. Please remember to bring your laptop (and charger).
  2. Please install the required software before the workshop - follow the installation instructions on this page.
  3. Download the workshop data by clicking on the link to the ZIP archive at the top of this page.
  4. You will be assigned to a specific group, with whom you will be sitting - your group number will be displayed in the training room.
  5. PLEASE CONTACT US BEFORE THE WORKSHOP IF YOU ENCOUNTER ANY DIFFICULTIES WITH ANY OF THE ABOVE.

Slides

All the slides for the workshop are on Google and accesible here.

Program

Please note that all catering, as well as attendance of the social on the evening of the 21st, is covered by your registration fee.

Monday, October 21st 2024
09:30 - 10:30 Registration
10:30 - 11:45 Session 1

Introduction - Automated Image Analysis is Essential for Reproducible Science

Dave Barry

  • Why manual analysis is a bad idea
  • Embracing uncertainty
  • What is metadata and why do you need it
11:45 - 12:00 Coffee Break
12:00 - 13:30 Session 2

Image Pre-Processing, Segmentation & Analysis

Dave Barry

  • Practical Applications:
    • Basic segmentation using thresholding
    • Use of filtering to suppress noise
    • Obtaining numbers from images
13:30 - 14:30 Lunch
14:30 - 16:15 Session 3

Assembling Pipelines and Interpreting Results

Dave Barry

  • Practical Applications:
    • Counting and quantifying morphology of objects
    • Quantifying fluorescence intensities
16:15 - 16:30 Coffee Break
16:30 - 18:00 Session 4

Introduction to Batch Processing

Dave Barry

  • Practical Application:
    • Record commands to form the basis of a script
18:00 - ? Evening Social The Lighterman, 3 Granary Square, N1C 4BH
Tuesday, October 22nd 2024
09:30 - 10:30 Session 5

Introduction to Python and installations

Stefania Marcotti

  • Creating Python environments
  • Installing Jupyter notebooks and napari
10:30 - 10:45 Coffee Break
10:45 - 12:30 Session 6

Using Jupyter Notebooks for Reproducible Analysis

Stefania Marcotti

  • Variables and arrays
  • Practical Application: quantify morphology of objects in a 2D image with Jupyter
12:30 - 13:30 Lunch
13:30 - 15:30 Session 7

Using napari as an image analysis tool and as a viewer

Martin Jones

  • Overview of napari GUI
  • Practical Application: batch processing with Jupyter
  • Practical Application: using napari as a viewer from Jupyter

Venue

The workshop will take place in the Francis Crick Institute, London, UK:

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Please note that the building entrance is on Midland Road.

Organisers

Previous Workshops

Date Venue Content
8 / 9th April 2024 King's College London Click here
24 / 25th April 2024 Royal College of Surgeons in Ireland Click here

FAQ

  1. Do I need any prior knowledge of image analysis, FIJI or napari to attend?

    No, this workshop is aimed at complete beginners, but a basic understanding of image acquisition would be beneficial.

  2. Do I need to have any experience of coding?

    While some basic knowledge would be helpful, it's not essential and even if you have no knowledge of python, Jupyter notebooks or FIJI scripts/macros, you should still apply.

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  • Jupyter Notebook 85.0%
  • ImageJ Macro 14.8%
  • SCSS 0.2%