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@@ -394,18 +394,27 @@ <h2>Study design</h2>
 (snRNA-seq) and spatially-resolved transcriptomics (SRT) data in the
 human hippocampus. (A) Postmortem human tissue blocks from the anterior
 hippocampus were dissected from 10 adult neurotypical brain donors.
-Tissue blocks were scored and cryosectioned for snRNA-seq assays (red),
-and placement on Visium slides (Visium H&amp;E, black; Visium Spatial
-Proteogenomics (SPG), yellow). (B) 10μm tissue sections from all ten
-donors were placed onto 2-5 capture areas to include the extent of the
-HPC(n=36 total capture areas), for measurement with the 10x Genomics
-Visium H&amp;E platform. (C) 10μm tissue sections from two donors were
-placed onto 4 capture areas (n=8 total capture areas) for measurement
-with the 10x Genomics Visium-SPG platform. (D) Tissue sections (2-4
-100μm cryosections per assay) from all ten donors were collected from
-the same tissue blocks for measurement with the 10x Genomics 3’ gene
-expression platform. For each donor, we sorted on both and PI+NeuN+
-(n=26 total snRNA-seq libraries). (This figure was created with <a href="https://biorender.com">Biorender</a>)</p>
+(B) Tissue blocks were scored and cryosectioned for snRNA-seq assays (gold),
+and placement on Visium slides (Visium H&amp;E, blue). (C) Top: Tissue sections
+(2-4 100μm cryosections per donor) from all ten donors were collected from the 
+same tissue blocks for measurement with the 10x Genomics Chromium 3’ gene 
+expression platform. For each donor, two samples were generated, one sorted
+ based on propidium iodide (PI, purple) and the second sorted based on PI+ and
+ NeuN+ (green). Replicate samples were collected from three donors for a total 
+of n=26 total snRNA-seq libraries. Bottom: 10μm tissue sections from all ten 
+donors were placed onto 2-5 capture areas to include the extent of the HPC 
+(n=36 total capture areas), for measurement with the 10x Genomics Visium-H&E 
+platform. Orientation was verified based on expression of known marker genes. 
+(D) Canonical marker genes were identified as spatially variable genes using nnSVG.
+(E) SRT data was clustered using PRECAST (30) with k=18 and clusters were annotated
+ (columns) based on expression of known marker genes (rows). Cluster groupings 
+indicated at the top of the heatmap define which clusters contributed to the 
+broad domains of Neuron, Neuropil, white matter (WM), and vascular/ cerebrospinal 
+fluid cell-enriched (Vasc/CSF). RHP: retrohippocampus, SUB: subiculum, CA2.4: 
+cornu ammonis (CA) regions 2 through 4 (CA2, CA3, CA4), GCL: dentate gyrus 
+granule cell layer, ML: dentate gyrus molecular layer, SL: stratum lucidum, 
+SR: stratum radiatum, SLM: stratum lacunosum-moleculare, SGZ: dentate gyrus 
+subgranular zone.</p>
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 <div id="interactive-websites" class="section level2">
 <h2>Interactive Websites</h2>