D3b/COV-IRT RNAseq SNV Calling Workflow (beta)
Pre-release
Pre-release
This workflow more closely follows how Broad runs in their WDL (no splitting of files nor intervals until haplotype call). Splitting (aside from haplotype calls) during bam pre-processing steps causes differences in overlap of calls and is not yet considered reliable without extensive benchmarking. It follows the COV-IRT pipeline except for gVCF generation. Preparation for joint calls is not part of this workflow and is more of a single sample call style.