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Investigating field of biology by applying logics of computer science, statistics and mathematics.
https://decodebiology.github.io
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Cold Spring Harbor Laboratory
- New York, US
- https://decodebiology.org
- @decodebiology
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TransExtract_betaV1.2
TransExtract_betaV1.2 PublicExtract Transitions from PARCLIP data. This is beta version, this can be improved by including more description about the results and also with graphical visualization.
Perl 1
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RNA-seq read count normalization (TP...
RNA-seq read count normalization (TPM and RPKM) 1#Rscript normalize_featurecounts.R counts_table.txt tpm ;
2#Rscript normalize_featurecounts.R counts_table.txt rpkm ;
34#Sample table###
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Check the Phred Scale quality of seq...
Check the Phred Scale quality of sequencing files 1#!/bin/bash
2# ./CheckPhredScale.sh <FASTQ_FILE> <NUMBER OF LINES TO CHECK>
3echo "Shell:";
45zcat $1 | head -n $2 | awk '{if(NR%4==0) printf("%s",$0);}' | od -A n -t u1 | awk 'BEGIN{min=100;max=0;}{for(i=1;i<=NF;i++) {if($i>max) max=$i; if($i<min) min=$i;}}END{if(max<=74 && min<59) print "Phred+33"; else if(max>73 && min>=64) print "Phred+64"; else if(min>=59 && min<64 && max>73) print "Solexa+64"; else print "Unknown score encoding";}';
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