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Caleb-Carr committed Jul 17, 2024
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2 changes: 1 addition & 1 deletion README.md
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# Deep mutational scanning of Lassa virus Josiah strain glycoprotein using a barcoded lentiviral platform
See [Carr, Crawford, ..., Bloom](https://www.biorxiv.org/content/10.1101/2024.02.05.579020v1) for the paper describing this study. Note that this repository has been updated since the release of the pre-print posted above so there could be small differences between the paper and pipeline figures.
See [Carr, Crawford, ..., Bloom](https://www.cell.com/immunity/fulltext/S1074-7613(24)00319-4) for the paper describing this study.

For documentation of the analysis, see [https://dms-vep.github.io/LASV_Josiah_GP_DMS/](https://dms-vep.github.io/LASV_Josiah_GP_DMS/)

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4 changes: 2 additions & 2 deletions homepage/.vitepress/config.mjs
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Expand Up @@ -12,14 +12,14 @@ export default defineConfig({
// https://vitepress.dev/reference/default-theme-config
nav: [
{ text: "Home", link: "/" },
{ text: 'Paper', link: "https://www.biorxiv.org/content/10.1101/2024.02.05.579020v1" },
{ text: 'Paper', link: "https://www.cell.com/immunity/fulltext/S1074-7613(24)00319-4" },
{ text: 'Cell Entry', link: '/cell_entry' },
{ text: 'Antibody Escape', link: '/antibody_escape' },
{ text: "Appendix", link: "/appendix", target: "_self" },
],
socialLinks: [{ icon: "github", link: "https://github.com/dms-vep/LASV_Josiah_GP_DMS.git" }],
footer: {
message: 'Study by <a href="https://www.biorxiv.org/content/10.1101/2024.02.05.579020v1">Carr, Crawford, et al (2024), bioRxiv, DOI 10.1101/2024.02.05.579020</a>',
message: 'Study by <a href="https://www.cell.com/immunity/fulltext/S1074-7613(24)00319-4">Carr, Crawford, et al (2024), bioRxiv, DOI 10.1101/2024.02.05.579020</a>',
},
},
});
2 changes: 1 addition & 1 deletion homepage/experiments_and_biosafety.md
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# Experiments and biosafety

The paper describing this study is [Carr, Crawford, et al (2024)](https://www.biorxiv.org/content/10.1101/2024.02.05.579020v1). The measurements are all made using a pseudovirus-based deep mutational scanning system that was originally described in [Dadonaite et al (2023)](https://doi.org/10.1016/j.cell.2023.02.001).
The paper describing this study is [Carr, Crawford, et al (2024)](https://www.cell.com/immunity/fulltext/S1074-7613(24)00319-4). The measurements are all made using a pseudovirus-based deep mutational scanning system that was originally described in [Dadonaite et al (2023)](https://doi.org/10.1016/j.cell.2023.02.001).

A key aspect of this system is that it utilizes pseudotyped lentiviral virions that encode no viral genes other than the viral entry protein (in this case GPC).
Pseudotyping is a [widely used and safe method](https://blog.addgene.org/viral-vectors-101-pseudotyping) to study viral entry proteins, since the pseudoviruses can only undergo a single cycle of infection and therefore pose no risk of transmission or replication in humans or any other animals.
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2 changes: 1 addition & 1 deletion homepage/index.md
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## About this site
This website contains interactive plots and numerical results from [pseudovirus deep mutational scanning](https://www.sciencedirect.com/science/article/pii/S0092867423001034?via%3Dihub) experiments that measure the effects of mutations to the glycoprotein complex (GPC) of the lineage IV Lassa virus Josiah strain on cell entry and antibody escape.

The paper describing this study is [Carr, Crawford, et al (2024)](https://www.biorxiv.org/content/10.1101/2024.02.05.579020v1).
The paper describing this study is [Carr, Crawford, et al (2024)](https://www.cell.com/immunity/fulltext/S1074-7613(24)00319-4).

The links in the boxes above take you to interactive plots or descriptions of different aspects of the study.
For a high-level overview, see the [summary](summary){target="_self"} of how mutations affect cell entry and antibody escape.
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