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Update README.md
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jjoung authored Dec 18, 2022
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Expand Up @@ -12,9 +12,9 @@ Scripts from the Joung et al Cell 2023 manuscript on a Transcription Factor Atla
- count_barcodes_10X.py counts TF barcodes from fastq
- tf_input_file: csv file of all possible TF barcodes with 1 barcode per line
- cell_input_file: csv file of all possible cell barcodes with 1 barcode per line
- r1_fastq_file: read 1 fastq file
- r2_fastq_file: read 2 fastq file
- output_prefix: prefix of files to write barcode count results to
- r1_fastq_file: read 1 fastq file
- r2_fastq_file: read 2 fastq file
- output_prefix: prefix of files to write barcode count results to
- map_cells_10X.py maps TF isoforms to cells using the counts_cells.csv file from the count_barcodes_10X.py output
- ORF_lib_barcode_map.csv: csv file of TF ORF barcode mapping to TF isoforms. Each line contains a TF barcode followed by the corresponding TF isoform
- counts_cells.csv: csv file of cell barcode mapping to TF ORF barcodes. Each line contains a cell barcode followed by TF barcodes and associated
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