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add viralrecon pipeline
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lpantano committed Jul 10, 2024
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## Nextflow in O2

### RNAseq

Example of running in single node Nextflow/nf-core/rnaseq in O2.

```
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export NXF_APPTAINER_CACHEDIR=/n/app/singularity/containers/shared/bcbio/nf-core-rnaseq-3.14.0
export NXF_SINGULARITY_LIBRARYDIR=/n/app/singularity/containers/shared/bcbio/nf-core-rnaseq-3.14.0
/n/app/bcbio/nextflow/nextflow run nf-core/rnaseq -r 3.14.0 -profile singularity,test --outdir here -resume
/n/app/bcbio/nextflow/nextflow run nf-core/rnaseq -r 3.14.0 -profile singularity,test --outdir this_folder -resume
```

### viralrecon

Read documentation [here](https://nf-co.re/viralrecon/2.6.0/).


This is an example for test data:

```
module load java/jdk-21.0.2
export NXF_APPTAINER_CACHEDIR=/n/app/singularity/containers/shared/bcbio/nf-core-viralrecon_2.6.0
export NXF_SINGULARITY_LIBRARYDIR=/n/app/singularity/containers/shared/bcbio/nf-core-viralrecon_2.6.0
/n/app/bcbio/nextflow/nextflow run nf-core/viralrecon -r 2.6.0 -profile singularity,test --outdir this_folder -resume
```
To run your data, prepare input file following this [doc](https://nf-co.re/viralrecon/2.6.0/docs/usage/#samplesheet-format), and run it like this:

```
/n/app/bcbio/nextflow/nextflow run nf-core/viralrecon -r 2.6.0 -profile singularity --outdir this_folder --input samplesheet.csv -resume
```


## Nextflow in FAS


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