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add parameters settings for us
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lpantano committed Nov 20, 2024
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Content - ![](https://img.shields.io/badge/status-WorkInProgress-yellow)

## Paramters

### RNAseq

- we use salmon with bam files mapped to transcriptome for quantification

### CHIPseq

- defaults parameters
- de-duplication for all samples
- bowtie is set up with these extra parameters: `--sensitive-local -X 1000`
- macs_gsize needs to be setup for each species accordingly ... tools

### CUT&RUN

- defaults parameters
- turn on dedup_target_reads

### ATACseq

All peaks `nf-core-atac-seq_shift`:
- shift is on
- keep_dup is false

NFR peaks `nf-core-atac-seq_shift_NFR`:
- same than previous except parameters for Aligmentsieve:
- `--minFragmentLength 0`
- `--maxFragmentLength 120`

## Nextflow in Seqera platform

- Create an user here: https://cloud.seqera.io/login
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