Update server_templates.R

[alexanderisme]

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[alexanderisme]

I added two CDS Entry plasmids as requested in #35
The plasmids are
pICH41308 for a CDS1
pAGM1287 for a CDS1ns

Hope someone can check if it is working as it is supposed.

Would it make sense to add all level0 acceptors described in the paper?
Engler C, Youles M, Gruetzner R, Ehnert T-M, Werner S, Jones JDG, Patron NJ, Marillonnet S. 2014. A Golden Gate Modular Cloning Toolbox for Plants. ACS Synth Biol 3:839–843. doi:10.1021/sb4001504

[sneumann]

That's great ! I'll setup a server with your code and come back to you. Yours, Steffen

[alexanderisme]

Dear Steffen,
I just noticed that for the "pAGM1287 for CDS1ns" there might be additional tweaks necessary since two base pairs have to be reintroduced in order to get the sequence of the C-Tag in frame again.
The "easy" solution would be to force a longer 3' overhang and use eg tcTTCG (reverse complemented = CGAAGA).
The elegant way would be to check if the sequence has or can be changed to a silent mutation introducing a T at the end of the sequence... But that would require more code and I am not able to do that.

Possibly the pAGM1287 for CDS1ns can be taken out for the moment in order to avoid confusion...

[sneumann]

Hi, thanks for your #37, which already addresses the difficult parts!
I only had to add that choice to the web interface. That version is now available for testing in
https://msbi.ipb-halle.de/GoldenMutagenesisWebDevel/
Could you please test-drive that ? Yours, Steffen

[alexanderisme]

Wonderful, everything looks perfect to me. Thank you very much!

[sneumann]

Does that also include I magically fixed #34 ? Yours, Steffen

[alexanderisme]

No, sadly that much magic happens only in Christmas time 😄
I was only referring to the overhangs formed by the pICH41308 vector...