-
Notifications
You must be signed in to change notification settings - Fork 4
Theory of single cell RNA seq
mAGLAVE edited this page Jan 19, 2024
·
9 revisions
Here are sources to understand the different steps and the biases of single-cell analysis:
-
Current best practices in single-cell RNA-seq analysis: a tutorial
-
Presentation of single-cell for biologists to initialize a project at Gustave Roussy (Github has trouble displaying the pdf correctly but you can download it, by clicking on the "Download" button)
-
Presentation of single-cell to present differences between LogNormalization and SCTransform (in French) (Github has trouble displaying the pdf correctly but you can download it, by clicking on the "Download" button)

Resources of the Theory of single cell RNA-seq
v1.3
Pipeline details
Configuration
-
Parameter file
- Steps
- Alignment_countTable_GE
- Droplets_QC_GE
- Filtering_GE
- Norm_DimRed_Eval_GE
- Clust_Markers_Annot_GE
- Cerebro
- Alignment_countTable_ADT
- Adding_ADT
- Alignment_annotations_TCR_BCR
- Adding_TCR
- Adding_BCR
- Int_Norm_DimRed_Eval_GE
- Int_Clust_Markers_Annot_GE
- Int_Adding_ADT
- Int_Adding_TCR
- Int_Adding_BCR
- Grp_Norm_DimRed_Eval_GE
- Grp_Clust_Markers_Annot_GE
- Grp_Adding_ADT
- Grp_Adding_TCR
- Grp_Adding_BCR
- Additional files
Results help
- Arborescence of all results
-
Observations and weird results
- Not a threshold by emptyDrops
- Large and small cells into the same sample
- emptyDrops does't work well
- More than 15% mitochondrial RNA while I filtered them out at 15%
- Impact of empty droplets on umap
- Choose the right number of dimensions
- Be careful with the colors, they are sometimes misleading
- Impact of bias correction on umap
Complete Examples of school cases
Individual analysis :
1 sample (scRNA-seq + ADT + TCR + BCR)
Grouped/Integrated analysis :
2 samples (scRNA-seq + ADT + TCR + BCR)
v1.3.1
Pipeline details
Configuration
-
Parameter file
- Steps
- Alignment_countTable_GE
- Droplets_QC_GE
- Filtering_GE
- Norm_DimRed_Eval_GE
- Clust_Markers_Annot_GE
- Cerebro
- Alignment_countTable_ADT
- Adding_ADT
- Alignment_annotations_TCR_BCR
- Adding_TCR
- Adding_BCR
- Int_Norm_DimRed_Eval_GE
- Int_Clust_Markers_Annot_GE
- Int_Adding_ADT
- Int_Adding_TCR
- Int_Adding_BCR
- Grp_Norm_DimRed_Eval_GE
- Grp_Clust_Markers_Annot_GE
- Grp_Adding_ADT
- Grp_Adding_TCR
- Grp_Adding_BCR
- Additional files
Results help
- Arborescence of all results
-
Observations and weird results
- Not a threshold by emptyDrops
- Large and small cells into the same sample
- emptyDrops does't work well
- More than 15% mitochondrial RNA while I filtered them out at 15%
- Impact of empty droplets on umap
- Choose the right number of dimensions
- Be careful with the colors, they are sometimes misleading
- Impact of bias correction on umap
Complete Examples of school cases
Individual analysis :
1 sample (scRNA-seq + ADT + TCR + BCR)
Grouped/Integrated analysis :
2 samples (scRNA-seq + ADT + TCR + BCR)